Protein-DNA Crosslinks

Chuck Miller rellim at MAILHOST.TCS.TULANE.EDU
Thu Mar 30 18:10:51 EST 1995

Hi Scott (s-ness at,

>Anyone know of an efficient method or reagent (other than UV) light to
>cross-link proteins (in a nuclear extract or even intact nuclei) to DNA?

Gamma radiation, formaldehyde and other aldehydes, and cis- or
trans-platinum compounds can be used to crosslink proteins and DNA in vitro
and in vivo.


Dr. Charles A. Miller 
Dept. Environmental Health Sciences  (374 CBR)
School of Public Health and Tropical Medicine
Tulane University Medical Center  
1430 Tulane Ave. Box SL29                 
New Orleans, LA 70112               
Ph. 504-585-6942                          
Fx. 504-585-6939
rellim at                                             

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