Protein-DNA Crosslinks

[Chuck Miller]

Hi Scott (s-ness at nwu.edu),

>Anyone know of an efficient method or reagent (other than UV) light to
>cross-link proteins (in a nuclear extract or even intact nuclei) to DNA?

Gamma radiation, formaldehyde and other aldehydes, and cis- or
trans-platinum compounds can be used to crosslink proteins and DNA in vitro
and in vivo.

Chuck

Dr. Charles A. Miller 
Dept. Environmental Health Sciences  (374 CBR)
School of Public Health and Tropical Medicine
Tulane University Medical Center  
1430 Tulane Ave. Box SL29                 
New Orleans, LA 70112               
Ph. 504-585-6942                          
Fx. 504-585-6939
rellim at mailhost.tcs.tulane.edu