Disulfide bonds

[Francesca Bahr]

                      Disulfide bonds
In article <3l8hse$sk at lyra.csx.cam.ac.uk>, cdw1000 at mole.bio.cam.ac.uk
(Christopher Walentas (Bioc)) says:
>
>Hi.
>
>I was wondering if anyone could share their favorite protocol for
>the oxidising of disulhpide bridges.

Make sure the pH of your solution is above the pKa of your Cysteines.  i.e.
For disulfide bond formation of heat-stable enterotoxin (6 cysteines!), we
used to  stir overnight  at pH 8.5 or so.

-Fran-

Bahr at ca.neb.com

============================================================================
  
             Francesca Bahr,   New England Biolabs Ltd,  Tel. 905-672-3370
             Internet: bahr at ca.neb.com                   Fax  905-672-3414