bahr at ca.neb.com
Thu Mar 30 11:28:57 EST 1995
In article <3l8hse$sk at lyra.csx.cam.ac.uk>, cdw1000 at mole.bio.cam.ac.uk
(Christopher Walentas (Bioc)) says:
>I was wondering if anyone could share their favorite protocol for
>the oxidising of disulhpide bridges.
Make sure the pH of your solution is above the pKa of your Cysteines. i.e.
For disulfide bond formation of heat-stable enterotoxin (6 cysteines!), we
used to stir overnight at pH 8.5 or so.
Bahr at ca.neb.com
Francesca Bahr, New England Biolabs Ltd, Tel. 905-672-3370
Internet: bahr at ca.neb.com Fax 905-672-3414
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