TA cloning : clone contamination
Thu Mar 30 08:24:56 EST 1995
I cloned a PCR product using TA cloning, I picked white colonies ,
analysed rapidly with PCR using the same primers I originally used for my
PCR product , they were all positive.
After O/N culture in liquid media, I did streak my white colonies,
surprisingly I found blue colonies growing on my plates that had X-Gal.
Is there any logical explanation for this apart from the very remote
possibility of contamination?Please help.
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