protein/polysach contaminants

M.DELAVINA at CGNET.COM M.DELAVINA at CGNET.COM
Thu Mar 30 05:48:38 EST 1995


i did some dna extraction two weeks ago and it seems that my dna product
has a lot of incorporated proteins and polysach.  these dnas are quite
precious since they're part of a mapping population and i can't mess them
up.  could anybody please recommend any protocol that could remove these
contaminants at the same time give me a good amount of quality dna. i've
tried adding a little sds and precipitating it with kac but it still did
not work.

any and all help would be appreciated. thanks!


Mercedes "Che" dela Vina
Int'l Rice Research Institute
PO 933 Manila
1099 Philippines

M.DELAVINA at cgnet.com




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