foote at wehi.edu.au
Thu Mar 30 00:28:19 EST 1995
Henry Keil (blsthhk at brunel.ac.uk) wrote:
: Hi everybody,
: we intend to carry out some YAC-fingerprinting on restricted
from chromosome 13 using IRS-elements as probes.
: We are not quite certain whether LINEs or Alu-repeats are the better source
for doing this experiment and what subtypes are most useful.
: Also does anyone know where one can obtain clones from which contain either
of these sequences as inserts.
Either work well, the difference is that you obviously see more bands if
an Alu probe is used and therefore the informative region of the gel is
that where there are fewer bands, i.e. among the smaller fragments wheras
L1's, being less abundant are informative across the entire range of
separable sizes. It is not necessary to use clones, you may use oligos.
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