mRNA in situ on sections with DIG-probe?

Christoph Grunau cgrunau at rkna50.riken.go.jp
Fri Mar 31 23:31:11 EST 1995


In article <Katarina.Nordqvist-3103951601130001 at chocolate.cmb.ki.se>,
Katarina.Nordqvist at mog.ki.se (Katarina Nordqvist) wrote:

> Hej!
> Does anyone have a nice protocol for doing mRNA in situs on sections of
> tissues with a digoxigenin (DIG, Boehringer Mannheim) labled RNA probe
> (the same kind of probe used for whole mount in situs). It would be nice
> to get hold of a protocol where it is possible to detect low expressing
> mRNAs.

Boehringer supplies a "Nonradioactive In Situ Hybridization Application
Manual" for free. May be that's of use for you.

Good luck - Christoph Grunau



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