mRNA in situ on sections with DIG-probe?

[Christoph Grunau]

In article <Katarina.Nordqvist-3103951601130001 at chocolate.cmb.ki.se>,
Katarina.Nordqvist at mog.ki.se (Katarina Nordqvist) wrote:

> Hej!
> Does anyone have a nice protocol for doing mRNA in situs on sections of
> tissues with a digoxigenin (DIG, Boehringer Mannheim) labled RNA probe
> (the same kind of probe used for whole mount in situs). It would be nice
> to get hold of a protocol where it is possible to detect low expressing
> mRNAs.

Boehringer supplies a "Nonradioactive In Situ Hybridization Application
Manual" for free. May be that's of use for you.

Good luck - Christoph Grunau