5'-RACE PCR for missing cDNA ends?

DREWES at MPASMB.DESY.DE DREWES at MPASMB.DESY.DE
Fri Mar 31 18:23:37 EST 1995


Hi Everybody -
I would like to hear about experiences with 5'RACE PCR cloning of cDNA ends,
since I isolated a couple of 5'-incomplete clones (as always??!)
from a library. My questions are:
      - Does it work generally well?
      - For what max. size of the missing 5'end does it still work ?
      - Is it better to make your own 5'-RACE cDNA (using a kit?) or
        to buy premade stuff from a company (Clontech) ?
      - What about mistakes due to PCR ampification?

*****************************************************************
*  Gerard Drewes                          DREWES at MPASMB.DESY.DE *
*  Max-Planck-Society                  or DREWES at CRYSTAL.DESY.DE*
*  Structural Molecular Biology Unit      Tel (+49)40-89982820  *
*  DESY, D-22603 Hamburg                  Fax (+49)40-891314    *
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