Labelling proteins in plant protoplasts

c515455 at c515455 at
Fri Mar 31 17:06:17 EST 1995

Does anyone have any suggestions for labelling proteins in plants protoplasts? 
 I've been trying to label a transiently expressed protein for the purpose of 
measuring its half-life.  Currently, I am pulsing the cells with 35-S 
methionine (100-200 micro CI) for 1 hr in a volume of 0.5 ml.  Are there 
conditions which would allow for more effectient uptake of the label?

Michael Curtin
Univ. of Missouri- Columbia
c515455 at

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