CsCl vs column kits
bkodrzyc at sumter.awod.com
Fri Mar 31 10:22:49 EST 1995
cynthia stryker (cstryker at unlinfo.unl.edu) wrote:
: We are still doing CsCl plasmid (&sometimes genomic) preps. I'd
: appreciate comments, + or -, on the various column kits available.
: Thanks in advance. Cindy at UNL
We switched from doing CsCl DNA preps to using QIAGEN columns for all of
our plasmid preps. The QIAGEN preps are easy and seem to give more
consistantly (high) yields of plasmid than CsCl. The DNA quality seems
to be good although sometimes I do phenol extract it after the final step
in the kit.
The elimination of Ethidium bromide from the DNA prep is a major plus.
Also there is no danger of sticking yourself with a needle (laced with
EtBr). The QIAGEN system works on gravity flow rather than
centrifugation and the columns are designed so that they don't dry out if
you forget about them. Thus it's possible to do you plasmid
purifications while you are doing something else without fear of messing up.
bkodrzyc at awod.com
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