CsCl vs column kits
Ian A. York
york at mbcrr.dfci.harvard.edu
Fri Mar 31 08:09:21 EST 1995
In article <0098E2DB.0E1EAB35 at nefeli.imbb.forth.gr> skoufos at nefeli.imbb.forth.gr writes:
>-you get more supercoiled DNA (essential if you are doing transfections)
I've heard this several times, but I haven't seen references or
experimental evidence for it. In the single trial I did (which was pretty
limited) I didn't see any difference between a Qiagen prep and CsCl prep
in a transient transfection of COS.
Is there a reference you can give me, or something, to back that up?
Ian York (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921 Fax (617)-632-2627
More information about the Methods