Methods March 1995 Archives by author

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Starting: Wed Mar 1 01:46:10 EST 1995
Ending: Fri Mar 31 23:41:52 EST 1995
Messages: 1461

One step transformation "Mayer, Jorge ", 3637
Nonidet P-40 "Mayer, Jorge ", 3637
plasmid nomeclature "Mayer, Jorge ", 3637
INValphaF' Juergen Sasse (Path)
Staining nitrocellulose *.*
DNA prep for transient transfection
Two-hybrid query
little blue homogenisers
Radioactive probes by PCR.
Stratagene's Quik Hybridization solution
Cellulose capsule removal from Acetobacter Labuser 1
test 190702082 Mcdonald, Ryan R.
ATCC e-mail ITABAJARA DA SILVA VAZ JUNIOR - 201
harvesting bone cells from ceramics POSTE 4
DNA elution from gels 4700gbera at umbsky.cc.umb.edu
Phenol distillation 4700gbera at umbsky.cc.umb.edu
Getting out the brown from DNA 4700gbera at umbsky.cc.umb.edu
Fructans and fructose? A.Marchant
Sea Urchin Eggs: Fertilization and PI cycle MICHAEL R ACAB
Urea SDS-PAGE AMCKINNE at UTKVX.UTCC.UTK.EDU
Anti P-Ser/P-Thr Antibodies AShaw5
Total cellular protein vs cell number AShaw5
Human Cerebellum and / or Mouse Cerebellum c-DNA Mr Hassan Abdulrazzak
Total cellular protein vs cell number Yasuhito Abe
immunofluorescence background Yasuhito Abe
(none) Sherif Abouelela
Pure bacterial chromosomal DNA Alejandro Abuin
IMPORTANT!! - MAIL QUEUE PROBLEMS AT NET.BIO.NET BIOSCI Administrator
UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ BIOSCI Administrator
IMPORTANT - UPDATE ON BIOSCI MAIL QUEUE PROBLEMS BIOSCI Administrator
IMPORTANT - BIOSCI MAIL QUEUE BACK TO NORMAL BIOSCI Administrator
Companies with E-mail/Gopher/WWW Mike Agostino
Introducing a single pyrimidine dimer into a DNA sequence Andrew Akerman
Calcium removal from Calmodulin Gabriel Akopian
DNA sequencing puzzle Alan
ECO-STAR John and Alex
Ligation problems John and Alex
Ligation problems John and Alex
Reference on urea breakdown John Altman
CD4 coding sequence- commercially available? Luis M. Alvarez
M-13 Purification kits Luis M. Alvarez
protein kinase A inhibitors Stephen Amato
Drosophila FM6 balancer problem Miss Hong Saw Tin Amy
Definition: Saturation Mutagenesis Eric C. Anderson
Strange PCR colony screen result Eric C. Anderson
Q: Direct PCR prod. sequencing Eric C. Anderson
Microwave techniques? Eric C. Anderson
sequence programs Eric C. Anderson
CsCl preps for ABI sequencing reactions Eric C. Anderson
address requested (fwd) Eric C. Anderson
Results: CsCl preps for ABI sequencing Eric C. Anderson
ABI 373 and PCR pdt Eric C. Anderson
suppliers of Fse1,Sse83871, BspG1 ??? Roger F. Anderson
Kits for genomic DNA from blood (lymphocy Annienell
M-13 Purification kits Annienell
To Dave Gilbert (sorry, others may ignore this) Tracy Aquilla
Disposable filters for buffers etc Tracy Aquilla
Phosphorimager Questions Tracy Aquilla
mouse vs human Tracy Aquilla
Does Klenow proofreading multiple-base 3' overhangs or just single base? Tracy Aquilla
Oligo 4.0-s program Tracy Aquilla
DNase treatment of RNA sample Tracy Aquilla
Request for actin clone (?) Tracy Aquilla
MW of IgG? Tracy Aquilla
phosphoimager Tracy Aquilla
PCR of yeast gDNA. John Aris
Human cDNA library in yeast expression vector. John P. Aris
Lac repressor-B-gal fusion protein? Greg Arnold
oocyte expression cloning Adam S. Asch
Antibiotic shelf life? Curt Ashendel
Glycerol shock in transfection-mechanism? William H Ashman
ECL problems Graham Atherton
Bcl-X antibody Graham Atherton
Microcentrifuge for Mol. Biol. -Best? Brick Ayola
Microcentrifuge for Mol. Biol. -Best? Brick Ayola
in vitro transcription/translation Brick Ayola
EtBr staining: A third option BCRUBIN at weizmann.weizmann.ac.il
Direct sequencing of PCR products BCRUBIN at weizmann.weizmann.ac.il
Need mice in Dallas, TX area GORDON BETTS
TRANSFER OF PLASMIDS INTO BACTERIA GORDON BETTS
TRANSFECTION OF PLASMIDS INTO E. COLI GORDON BETTS
SKILLS REQUIRED BY CORPORATE LABS GORDON BETTS
DNA Strider GORDON BETTS
Restriction Digestion and Bacteria GORDON BETTS
Research mice & where should they come from? GORDON BETTS
Journal Table of Contents GORDON BETTS
TRANSFER OF PLASMIDS INTO BACTERIA BIO101 at DELPHI.COM
Large Scale Insect Cell Culture BIOTEC54
Bal31 source BIOTEC54
Cell line expressing sLex antigen BMA14432 at vax1.utulsa.edu
How many DNA binds to Nylon membranes Dietmar Baas
DNA comparison Gbolagade Babalola
Disulfide bonds Francesca Bahr
M-13 kits Claude Baker
M-13 Kit Claude Baker
M-13 Purification kits Claude Baker
HYbridization Incubator Simon Baker
UK supplier of Seaplaque GTG LMP agarose Simon Baker
Freezing Bacteria-Liquid N2 OK? Kimberly Baldwin
microbiocides Hendrik Ballerstedt
culturing spermatozoa Harry J. Banaharis
Unsubscribe Banstead at aol.com
Favorite bacterial RNA prep?? Melanie Jean Barnett
why fix sequencing gel? Kirk Bartholomew
NIH Image Dr. Robert Bateman
Dyes and supercoiled DNA Andy Bates
Ligation of alkaline phosphatase treated DNA Andy Bates
GFP Dave Bates
Transfection efficiency Dave Bates
Hemoglobin fluorescence Dave Bates
Hemoglobin fluorescence Dave Bates
Nonidet P-40 Lara Endres Baxley
why fix sequencing gel? Anne Bazanowski
Direct fluorescein labeling of DNA Edward Beaty
pLysS freezer stock instability Edward Beaty
Toothpick sources in the UK Edward Beaty
TAQ genomic library?? Wendy Bedale
Autoclaving Dextran Sulfate? Dr Joseph Behnam
Microwave techniques? Scott Belcher
electrophorisis of double nicked circle Andrew Belt
antisense oligonucleotides M K Bennett
Temp. Gradient Gel Electrophoresis/GC clamps M K Bennett
Temperature gradient gel electrophoresis (TGGE) M K Bennett
Methods shortcourse? Allan Berger
Metabolic pathways poster? Andrew Berry
Acetyl Coenzyme A Michael Berryhill
Software for oligo Thierry Berthe
RT-PCR with Dynabeads Roger Besançon
Oligo 4.0-s program Lisa Bibbs
ABI 373 and PCR pdt Lisa Bibbs
alfalfa mosaic virus enhancer? Bill
y Bill
oxidation of SS bridges Christopher Walentas Bioc
E.coli "gene knockout": help Robert Solomon Bioc
apoptosis detection: problems with test kits Inst. for Biochemistry
Phenol distillation Shaun D. Black
Journal Table of Contents Shaun D. Black
Fisher Scientific Shaun D. Black
Toothpicks for PCR Darren Boehning
Running Retic lysate on SDS-PAGE Darren Boehning
detecting pcr-products by hplc Holger Bohnemeier
little blue homogenisers Peta C. Bonham-Smith
ATCC Catalogs? Torsten Borchers
alternative to silver stain? Torsten Borchers
E.coli "gene knockout": help Giuseppe Borsani
Promoter recognition and Alu masking Giuseppe Borsani
Promoter recognition and Alu masking Giuseppe Borsani
looking for a rat genomic library Giuseppe Borsani
4-aminobutyric acid assay Mohohlo Molatudi Botany
test Mohohlo Molatudi Botany
4-aminobutyric acid Mohohlo Molatudi Botany
Gentra Systems kits Ian A. Boussy
Electrophoresis Gel? Chris Boyd
Toothpick sources in the UK Chris Boyd
little blue pestles Paul D. Boyer
Problems with densitometry!....for WESTERN BLOTS Paul D. Boyer
Microwave techniques? Scott K. Boyer
0.2-mL PCR tubes John Braverman
Leukemia Res. Conf. in Chicago (9/95) Eric Bremer
Okazaki fragments -- in Thermicus aquaticus? Rob Britton
Oligo Question Rob Britton
Trizol for total RNA M.Johan Broekman
FOR SALE: Discount Lab Glassware John Brookes
Help! RNA extraction Andrew Brooks
RE map software Karen Browning
Antibiotic shelf life? John Brunstein
SURE vs. NovaBlue competent cells John Brunstein
Bal31 source John Brunstein
Phosphorimager Questions John Brunstein
unexplained insert from PCR clone? Steve Bryson
best immunogen, injctn. schedule for rabbit Paul A Bucciaglia
green fluorescent proteins William J. Buikema
PCR detection of viral RNA in whole blood specimens Paul Bumbak
PCR detection of viral RNA in whole blood specimens Paul Bumbak
PCR detection of viral RNA in whole blood specimens Paul Bumbak
Electroporation of Antibodies Dan Burgess
P1 library Screening Daniel L. Burgess
mouse vs human Daniel L. Burgess
phosphorothioate oligos for antisense Jim Burrows
Vent DNA polymerase and asymmetric PCR Tim Buss
GAP genomic sequences? C.J. Hussussian, MD
wierd cell culture contam C.J. Hussussian, MD
ssDNA synthesis CVLowry
SURE vs. NovaBlue competent cells Malcolm Campbell
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES Malcolm Campbell
Infection with nematode eggs Brendan Carson
biotin background problem Diogo Cavaco
MULV-RT Ist. Cibernetica - Rep. Sistemi Cellulari
Looking for human aFGF and bFGF cDNA clones Dr. C.J. Chan
want an ABI 380B DNA synthesizer Jeffrey Chang
Postdoctoral Position Tien-Hsien Chang
STR systems Wasun Chantratita
Attachment of primary cell cultures Fengling Chen
antibody against fibronectin receptor Shao-Yu Chen
lambda insert isolation Cheung Cho Yue, M.D.
In situs in Drosoph. saliv. C. Chihara
Fluoro-dATP sequencing kit problem Casey Chin
phosphoimager Fung Ming Chiu
phosphoimager Fung Ming Chiu
in situ labs? Christiane=Frahm at rz.hu-berlin.de
molecular modeling software Gayle Christopher
Bal31 source Dr I.M. Clark
Rhodamine-4-dUTP and biotin-11-dUTP Duncan Clark
Pfu/Taq in PCR reactions Duncan Clark
Okazaki fragments -- in Thermicus aquaticus? Duncan Clark
Okazaki fragments -- in Thermicus aquaticus? Duncan Clark
suppliers of Fse1,Sse83871, BspG1 ??? Duncan Clark
Curing E.coli plasmids Duncan Clark
Direct selection for GalE in E.coli Duncan Clark
Direct selection for GalE in E.coli Duncan Clark
Msp1 Genomic Digests Duncan Clark
E. coli W3110 not accepting two plasmids Duncan Clark
BbvII Restriction enzyme Duncan Clark
LacZ Xsomal deletions Duncan Clark
Toothpick sources in the UK Duncan Clark
Toothpick sources in the UK Duncan Clark
Fisher Scientific Duncan Clark
Northern Red Shrimp/Pandalus borealis Duncan Clark
NIH Image Duncan Clark
pWE15 Cosmid vector D L Clarke
pWE15 Cosmid vector D L Clarke
SYBR and gel staining Ian Clements
size determination of rflps Dr. J.P. Clewley
oocyte expression cloning Michael Coady
EDTA in hybridization? A. F. Cockburn
Changing RE to T/A Randall J. Cohrs
human neurofilament (NF-H) Randall J. Cohrs
Microwave techniques? Randall J. Cohrs
non-palindromic RE? Randall J. Cohrs
PCRs no longer working. HELP! John Compton
DNA Strider Question Rick Conrad
Question: What does BSA do in PCR reactions? Rick Conrad
EtOH precipitation Rick Conrad
Nucleic acid modifications Rick Conrad
Poser for PAGEophiles Rick Conrad
Problems with yeast two-hybrid system Brian M. Cooke
Enhancement of PCR? Michael Cooley
CAT assays in E. coli Michael Cooley
Toothpicks for PCR Michael Cooley
shake after electroporation? Michael Cooley
Molar extinction coefficient of a protein Alan Cooper
MAKING YOUR OWN GEL BOXES Seth Corey
Accession numbers for human JAKs and STATs Seth Corey
transient transfection Seth Corey
Yeast 2-Hybrid Seth Corey
yeast two-hybrid Seth Corey
CALL: Peer Review Panel Recruitment: American Institute of Biological Sciences Keith L. Cowing
Transfecting Primary Cell Lines Dermot Cox
CAT assays in E.coli Albert Crescenzo
CAT assays in E. coli Albert Crescenzo
Help!PCR screening protocol instead of miniprep Emma Croager
Mitochondrial DNA inheritance Hector Cruz-Lopez
Membrane oxidoreductase on sperm? Jim Cummins
need hsp70 Sandy Curtiss
D-aspartate in bacteria Marc DIEU
Specificity of endoproteinase Glu-C Marc DIEU
Molar extinction coefficient of a protein at 280 nm. Marc DIEU
Kinase Substrate Sequences DREWES at MPASMB.DESY.DE
ds- versus ss-DNA sequencing DREWES at MPASMB.DESY.DE
Gamma-Phosphate linked ATP-Sepharose (UBI) for purifying protein kinase DREWES at MPASMB.DESY.DE
5'-RACE PCR for missing cDNA ends? DREWES at MPASMB.DESY.DE
Msp1 Genomic Digests DYDM6025 at iruccvax.ucc.ie
Church Buffer, high background DYDM6025 at iruccvax.ucc.ie
Phenol distillation Roy Dace
GeneClean vs Eluquik vs Prep-a-gene R Daines
pcDNA3 R Daines
Microwave techniques? Mike Dalrymple
Need DNA method for algae Ambiocom - Richard Dana
Algae DNA Ambiocomm - Richard Dana
Getting out the brown from DNA Richard Dana
SV40 Daniel, Phil
Nonidet P-40 Steve Danko
EtBr in agarose gels vs. EtBr staining later David L. Haviland, Ph.D.
Epitope tagged molecules David L. Haviland, Ph.D.
Q:Does Gene Clean interfere with ligation? Simon Dawson
Two-hybrid query Simon Dawson
Oligo 4.0-s program Simon Dawson
Why can't I get agarose embedded HMW DNA to digest ? Todd DeZwaan
HELP: Microtubuli or tubulin Todd DeZwaan
Site-directed Mutagenesis Oligos Todd DeZwaan
Rapid Polyacrylamide Polymerization Victor Defilippis
GST-Fusion R. Rex Denton
Hybridization oven Theo Devos
Disposable filters for buffers etc Dan Diaz
WHO SELLS EXO-KLENOW??? Dan Diaz
How to get rid of excess adaptors? John Dixon
microwave digests (hmmm) John Dixon
Will I lose half my adaptor by gel purifying? John Dixon
Southern with small probe? John Dixon
Microwave techniques? John Dixon
CsCl preps for ABI sequencing reactions John Dixon
An enzyme degrading dsDNA but not ssDNA John Dixon
freq of amber revertants in lambda ZAP? John Dixon
Invitrogen SP6 and T7 primers... John Dixon
Invitrogen SP6 and T7 primers... John Dixon
Taq-Pfu compatibility Tom Doak
Univ. charging $100/mo./person for mainframe access Donald A. Lehn, Ph.D.
shake after electroporation? Qunfeng Dong
detecting pcr-products by hplc Peter Doris
Ghost bands on Western blot? Mr TG Downing
Mobility Shift and Glutamic Acid Ken Doyle
suggestions on automated DNA sequencer? Hong Yan Dr
address of Molecular Probes Inc. ?? Dr.
CO2 Cell Culture Incubator Virginia Dress
DNase treatment of RNA sample Virginia Dress
Phosphorylated amino acids? Dynagen at rt66.com
tissue culture cell membrane proteins George F Edick
PCR sux's Clinton Edwards
PCR sux's Clinton Edwards
Re-using hybridisation solutions containing double-stranded probes? Jan Eggermont
Transfecting Primary Cell Lines Jan Eggermont
antisense oligonucleotides Jan Eggermont
Subcloning large fragments Nick Ellison
anti-murine TGFbeta2 antibody Torreele Els
Transfecting Primary Cell Lines Emmanuel Skoufos, Ph.D.
mouse vs human Emmanuel Skoufos, Ph.D.
Promoter recognition and Alu masking Emmanuel Skoufos, Ph.D.
transient transfection Emmanuel Skoufos, Ph.D.
X-ray film storing Emmanuel Skoufos, Ph.D.
Antibiotic shelf life? Emmanuel Skoufos, Ph.D.
Ligation problems Emmanuel Skoufos, Ph.D.
RNAse protection assay Emmanuel Skoufos, Ph.D.
Site-directed Mutagenesis Oligos Emmanuel Skoufos, Ph.D.
CsCl vs column kits Emmanuel Skoufos, Ph.D.
CO2 Cell Culture Incubator Cheah Kathryn Song Eng
MJ PCR machines and BioRad gel doc systems Cheah Kathryn Song Eng
ligation/transformation: HELP ME PLEASE!! Shin Enomoto
MEDLINE. David J. Evans
Accession numbers for human JAKs and STATs FNINER
Enzyme-chimeras FNINER
Nucleic acid/protein sequence analysis software for Mac FNINER
RNA isolation from cryostat section KOH FURUTA
RNA isolation from cryostat section KOH FURUTA
DMSO in PCR rxns ?? Glibert Fabrice
DNA sequence puzzle! Tom Fagan
test, disregard Robert Farese
tissue culture cell membrane proteins Mark Farinha
Denaturing ds DNA for sequencing Mr SMNN Faruque
alignment software Mr SMNN Faruque
protein marker for insect microsomes Darren Fast
Ghost bands on Western blot? Darren Fast
"missing" RNA Karen Ferri
gene expression in neurons Fiberman
antibody compatibility Fiberman
storing western blots Fiberman
CREB antibodies Fiberman
Church hyb solution questions Edwin Fink
Biotinylation of bradykinin Edwin Fink
antisense oligonucleotides Gavin Fischer
On/Off rates of Rec. Fabs? H. David Fischer
GPI anchors H. David Fischer
Pischia expression H. David Fischer
RNA from Liver Karl Fischer
Microcentrifuge for Mol. Biol. -Best? Karl Fischer
SDS Removal/hemolytic protein Karl Fischer
Formaldehyde RNA gels. Karl Fischer
Site-directed Mutagenesis Oligos Karl Fischer
RNA from Spleen - Help! Karl Fischer
Where to get Nonidet P-40 Karl Fischer
An enzyme degrading dsDNA but not ssDNA Paul Robert Fisher
MULV-RT Parke K. Flick
Taq extenders and Pfu polymerase Parke K. Flick
WHO SELLS EXO-KLENOW??? Parke K. Flick
address of Molecular Probes Inc. ?? Parke K. Flick
YAC fingerprinting Simon Foote
eosophageal cells John Fox
eosophageal cells John Fox
DNA Preparations John Fox
MeOH in Western Buffer John Fox
Electroporation John Fox
DTT in in vitro transcription Rx John Fox
transfection quality DNA- help John Fox
Favorite bacterial RNA prep?? John Fox
0.2-mL PCR tubes John Fox
Electroporation of Protoplasts John Fox
Rates of DNA oligo annealing Michael P. Frank
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES John M. Freeman
2-D Electrophoresis P. Friedl
Analysis of 2D-gels with scanner and PC P. Friedl
Transiet transfection of neurons Matthew Frosch
T7 RACE? Mark Fry
Optimum temp for PCR primer annealing Hui Yuk Fun
RNA isolation from cryostat section Koh Furuta
G0 antibody Koh Furuta
heteroplasmy of cloning vectors G
Enriching gene copy no. G
OSTEOPONTIN and OSTEOCALCIN antibodies commercially available? G.E.Fazzi
little blue homogenisers GANESHAN at SASK.USASK.CA
smearing on pulse field gel GEN282E at ccs1.cc.monash.edu.au
Curing E.coli plasmids Tamas Gaal
Curing E.coli plasmids Tamas Gaal
Curing E.coli plasmids Tamas Gaal
Depurinate & Break DNA? Miguel Angel Rodriguez Gabriel
plasmid isolation from yeast Marco Gagiano
pfu polymerase Michael Gale
TG repeats (microsatellites) G Gallagher
Church hyb solution questions Warren Gallin
Total cellular protein vs cell number Warren Gallin
Differential Display Olivier Gandrillon
Sokolov/Prockop DDRT Olivier Gandrillon
/equipment/ABI380B DNA SYN. John Gardner
Analysis of 2D-gels with scanner and PC John M. Garrison
Total cellular protein vs cell number Stephen Gately
Ligation problems Stephen Gately
Retrieval of ssDNA from a mixture of ssDNA/dsDNA Allen Gathman
Optimum temp for PCR primer annealing Allen Gathman
Optimum temp for PCR primer annealing Allen Gathman
Hyg assays in plants Jutta Tuerck Genetics
mutanolysin Szatmari George
storing western blots Gerard
Problems with densitometry!....for WESTERN BLOTS Gerard
HELP! Need enzyme (cyclophilin) fast J.P. Germanas
Autoclaving Dextran Sulfate? Alan Gerstein
PCR amplification of cloned insert Gil
immunofluorescence background Dave Gilbert
Info on Santa Cruz Biotechnology Giwamot at indycms.iupui.edu
PCR Product Purification for Seq? Greg Gloor
Phosphorimager Questions David Glover
formaldehyde pH David Glover
degenerate primers PCR Steven Goldberg
DNA Strider Marc Goldstein
Two hybrid Q. Erica Golemis
electrophorisis of double nicked circle David Gonda
Storage of PEG solutions Liam E Good
PCRs no longer working. HELP! Jim Gore
Hemoglobin fluorescence David S. Gottfried
PCR primer help! Wayne Gottlieb
TA cloning kit (In Vitrogen) Wayne Gottlieb
Thioredoxin fusion expression system Kathryn Graham
PCRs no longer working. HELP! Dawn Gray
PCRs no longer working. HELP! Dr Keith Grimaldi
PCR & spermidine Dr Keith Anthony Grimaldi
Genomic DNA from cells (re: Kits) Dr Keith Anthony Grimaldi
Gentra Systems kits Charles M Griswold
progesterone responsive rat RNA probe Mike Gruidl
again polystyrene biodegradation Christoph Grunau
CLONING PCR PRODUCT Christoph Grunau
mRNA in situ on sections with DIG-probe? Christoph Grunau
Transforming E. coli with Yeast DNA... HOW!? Esther Guarinos
SV40 HANELT
ligation/transformation: HELP ME PLEASE!! HARDIES at THORIN.UTHSCSA.EDU
Tm of a DNA hairpin HARDIES at THORIN.UTHSCSA.EDU
PCR with 500bp Primer HARDIES at THORIN.UTHSCSA.EDU
Enhancement of PCR? (by boiling primers) HARDIES at THORIN.UTHSCSA.EDU
ligation/transformation (type I restriction) HARDIES at THORIN.UTHSCSA.EDU
la pcr complexes (a network) HARDIES at THORIN.UTHSCSA.EDU
help me!! (254 nm UV damages DNA) HARDIES at THORIN.UTHSCSA.EDU
Oligo 4.0-s program (how to adjust parameters) HARDIES at THORIN.UTHSCSA.EDU
ligation problems HARDIES at THORIN.UTHSCSA.EDU
electrophorisis of double nicked circle HARDIES at THORIN.UTHSCSA.EDU
suppliers of Fse1,Sse83871, BspG1 ??? HARVEY
(none) HOWARD at MGRP.BGSM.WFU.EDU
Legal Polymerase?? HOWARD at MGRP.BGSM.WFU.EDU
Wanted Verticillium agaricinum Anthony Hall
In Situ Hybridization Brian Halloran
mol genet forensic "bible"? Andre Hamel
small microfuges/quick spins? Andre Hamel
PCRs no longer working. HELP! Andre Hamel
Bacteriophage Strain Collection Paul Hamilton
Q:Does Gene Clean interfere with ligation? Richard R. Hardy
immunofluorescence background Richard R. Hardy
differential display Richard R. Hardy
transfection of keratinocytes Alexis Harington
Misincorporation rates of polymerases Uwe Harmening
Plasmid Diagrams Neil Harris
M-MLV Neil Harris
Anion/Cation free agar Neil Harris
Cryptic splicing of mammalian cDNA in transfected TC cells Harry_Au at SOM-LRC.UCSD.EDU
in vitro transcription/translation Yasha Hartberg
RFLP subtraction Takashi Hashimoto
A beginner in RACE PCR, or possibly inverse PCR needs advice! Richard Hastings
Enhancement of PCR? Richard Hastings
Church for Northern blots? Randy Haun
Any pACYC plamids with T7 promoters? Randy Haun
COS-1 cells wanted Randy Haun
Differnetial Display Don Haut
P1 Screening/isolation Amos Heckendorf
Help needed-- understanding POROS / Perfusion chromatography Amos Heckendorf
Transfections using QIAGEN Amos Heckendorf
How to get rid of excess adaptors? HedstromR
Phosphorimager Questions Peter Heifetz
digital gel documentation Peter Heifetz
Riboflavin in Acrylamide Scott Selby Med Heme
Gentra Systems kits Scott Selby Med Heme
2-Mercaptoethanol, thanks Paul N Hengen
Pfu/Taq in PCR reactions Paul N Hengen
EtBr in partial digests of DNA Paul N Hengen
Wooden Toothpicks (Taq Inhibitor) Paul N Hengen
FTP site for FAQ list and TIBS manuscripts restored Paul N Hengen
ASCII file of molecular biology words Paul N Hengen
TA cloning -Promega Paul N Hengen
EtBr decontamination Paul N Hengen
Microwave techniques? Paul N Hengen
DNA sequence puzzle! Paul N Hengen
storing western blots Paul N Hengen
storing western blots Paul N Hengen
PCR amplification of cloned insert Paul N Hengen
Where to get Nonidet P-40 Paul N Hengen
Frequently Asked Question Pointer Paul N Hengen
Where to get Nonidet P-40 Paul N Hengen
Protocol for downward blot Paul N Hengen
Taq extenders and Pfu polymerase Your Name Here
random hexamers as primers for RT in RT-PCR Robert Hewitt
Killer gene Bernard Heymann
Wax beads Jody k. Hirsh
Plasmid Diagrams Tony Hodge
RNA from spleen Chris Hodgson
Microwave techniques? John Hodgson
Anyone using Ericomp thermal cyclers? John Hodgson
software to generate restrcition fragment graph John Hodgson
Urea SDS-PAGE Brian A. Hollander
Antibody for detecting murine mdm-2 Annette C. Hollmann
MeOH in Western Buffer Louis Hom
Request for Site directed mutagenesis Protocols (PCR) Hooperkp
PCR CLONING AND FASLE WHITE COLONIES Christopher Hoover
Replica plating of cells Peter J. Hornsby
Vent DNA polymerase and assymmetric PCR Robert Horton
GCG manual Robert Horton
Wax beads Robert Horton
How do you calculate annealling temperature if DMSO added??? Bixing Huang
GST-Fusion I-Jen Huang
How do I make a TA cloning vector? Mike Hubank
Nla IV - Restriction Enzyme Tim Hutchin
DNA FROM WHOLE BLOOD I.Ridgers at nhm.ac.uk
GFP in CHO cells ICAgen
CD4 coding sequence- commercially available? ICAgen
Where to get Nonidet P-40 ICAgen
Stratagene's Quik Hybridization solution Jun IKEDA
Genetic Diagnostics/Infectious Diseases Conference Cambridge Healthtech Institute
DNase treatment of RNA sample Toshiharu Ishizuka
DNase treatment of RNA sample Toshiharu Ishizuka
anti-centromere antibody? source? J.Carnwath
EtBr , How do you get rid of it? J.Carnwath
Phenol distillation JAG at QUCDN.QueensU.CA
Trouble with Fisher brand cartridge for Nanopure JAG at QUCDN.QueensU.CA
SDS Removal JFigue
TAQ ERROR RATES JIM
Want PCR to incorporate 32P label PAUL JONES
AZTREONAM, HELP! Jack M. Bernstein, M.D.
MW of IgG? Jack M. Bernstein, M.D.
Recombinant Circle PCR Don Jackson
GAL4 Antibodies? Don Jackson
(none) Sei-Heon Jang
SYBR Green Jfloring at aol.com
cosmid subcloning William Jia
Peptides on the head of a Pin? JoannePetithoryjpetithory at genencor.com
(none) Michael Johns
Gel box less than 1 dollar Nsubuan Johnson
Idaho Thermocycler Todd Johnson
Antibiotic shelf life? William C. (Bill) Johnson
Metabolic pathways poster? (Dave Johnston)
CO2 Cell Culture Incubator (Dave Johnston)
Alternatives to Formalin/Formaldehyde? (Dave Johnston)
Source of thin walled tubes (U.K) (Dave Johnston)
little blue pestles Jillian Johnston
DNase treatment of RNA Jillian Johnston
non-palindromic RE? Jillian Johnston
C-terminal sequencing? Mandy Johnstone
Source of thin walled tubes (U.K) Chris Jones
pcDNA3 Mick Jones
Okazaki fragments -- in Thermicus aquaticus? Zophonias O. Jonsson
alignment software Zophonias O. Jonsson
recombinant protein purification Zophonias O. Jonsson
Complement and plastic microtiters Luca Jovine
Phosphorimager Questions Malcolm Moos Jr.
alternative to silver stain? Malcolm Moos Jr.
MeOH in Western Buffer Malcolm Moos Jr.
PCR using lambda gt11 primers Malcolm Moos Jr.
unsubscribe KARN at Butler.EDU
*** Unscheduled DNA synthesis? *** KGEISS%RAVEN at EAGLE.AAMRL.WPAFB.AF.MIL
pedigree software JOHN A. KORTE
*** Unscheduled DNA Synthesis? *** KTGEISS at MIAVX1.ACS.MUOHIO.EDU
Squalene/H2O2 reaction - study.txt [1/1] Ted Kalli
Chemical Cleavage of Proteins/Peptides Angeline Kantola
wierd cell culture contam Jason Kantor
Plaque PCR mystery Wen-Yen Kao
Chemical Cleavage of Proteins/Peptides Wen-Yen Kao
Mab for OspA of Borrelia???? Ali Karami
Mab for OspA of Borrelia???? Ali Karami
Introducing a single pyrimidine dimer into a DNA sequence Scott Keeney
Source of thin walled tubes (U.K) Keith
Thin-walled PCR tubes (UK) Keith
long range PCR Keith
re noniodet P40 Keith.Wright at WPO4.CL.HealthWaikato.HWL.synet.net.nz
Monteray GFP meeting Kendall
Ligation of alkaline phosphatase treated DNA Martin Kennedy
Microwave techniques? Martin Kennedy
Microwave techniques? Martin Kennedy
phosphorothioate oligos for antisense Dave Keys
MAKING YOUR OWN GEL BOXES Daniel Kim
small microfuges/quick spins? Daniel Kim
Restriction enzymes and methylation Daniel Kim
Where to get vector phGHam-g3? Daniel Kim
Recombinant Circle PCR Daniel Kim
The One-dollar Gel Box Daniel Kim
MeOH in Western Buffer Daniel Kim
Stratagene SurfZAP phage display vector Daniel Kim
Need connexin 26 antibody Dooyeon Kim
Need help on RNA isolaton from plant Soo-Hwan Kim
Riboflavin in Acrylamide Klaus.Matthaei at ANU.EDU.AU
BbvII Restriction enzyme Klaus.Matthaei at ANU.EDU.AU
Oligo 4.0-s program Klaus.Matthaei at ANU.EDU.AU
Ligation problems Klaus.Matthaei at ANU.EDU.AU
DNA-Protein interactions Handbook Ralf Kleyer
ANNOUNCE: NEW WEB SITE - World Health Net Eric Klien
ANNOUNCE NEW WEB SITE: World Health Net Eric Klien
Anti P-Ser/P-Thr Antibodies Stephan Klinz
Any one used Oligos from Universal DNA Inc? $1/base! Michael Kloth
CsCl vs column kits Bob Kodrzycki
Ligation of large plasmids Bob Kodrzycki
MW of IgG? Steve Konings
Precut nylon hybridization membranes? Jarkko Kortesmaa
Fluoro-dATP sequencing kit problem Jarkko Kortesmaa
Old World Monkey cell line sources? Paul Edward Kowalski
farnesyl transferase (Piotr Kozlowski)
(none) Jeff Kramer
mouse vs human Jeff Kramer
transcription inhibitors Jeff Kramer
(none) Sergei Krasnyanski
(none) Sergei Krasnyanski
Oligo Question Andy Kravetz
CsCl vs column kits Andy Kravetz
Univ. charging $100/mo./person for mainframe access Candace Krepel
HELP ME!! (lig/trans: UV and CIP) -- repeat message Joel A. Kreps
DNA-Protein interactions Handbook Georg Kroeger
M13 ssDNA degradation? Dubear Kroening
M13 ssDNA stability Dubear Kroening
green fluorescent proteins Stuart Kuhstoss
Pischia expression Jonathan Kurtis
control for TPA induction L.Y.Chau
Ab for ferritin? L.Y.Chau
sub. of plant tissue culture newsgroup LEES at SASK.USASK.CA
Microwave techniques? LEES at SASK.USASK.CA
address requested (fwd) LEES at SASK.USASK.CA
looking for address (fwd) LEES at SASK.USASK.CA
Rf: Metabolic pathways poster? LOGAND
Rf: Precut nylon hybridization membranes? LOGAND
Rf: Re: Enhancement of PCR? LOGAND
Rf: How "random" are random primers? LOGAND
Random hexamers (again) LOGAND
Sticky DNA after Hind III-Why? LOGAND
Nicotiana "gene banks" LOGAND
Source of SceI LONSDALE
Inosine in degenerate PCR primers? Hancock Lab
Help! Unexplained deletion in gene Ramkumar Lachumanan
Research mice & where should they come from? John Ladasky
Univ. charging $100/mo./person for mainframe access Carlisle Landel
Is it Taq or the primer responsible for the mistake? Carlisle Landel
WANTED: Course lab protocols Garry P. Larson
VNTR loci analysis using Genescan Garry P. Larson
Phylogenetic tree construction Garry P. Larson
Subcloning large fragments Susini Laurent
Q : E. coli instability Elizabeth Lawrence
Metabolic pathways poster? Martin D. Leach
Transfecting Primary Cell Lines Martin D. Leach
TRANSFER OF PLASMIDS INTO BACTERIA Martin D. Leach
In vitro transcription ...... Martin D. Leach
TRANSFECTION OF PLASMIDS INTO E. COLI Martin D. Leach
Companies with E-mail/Gopher/WWW Martin D. Leach
DNA prep for transient transfection Martin D. Leach
who sells cheap MOPS. PLEASE.****** Martin D. Leach
Precut nylon hybridization membranes? Martin D. Leach
Precut nylon hybridization membranes? Martin D. Leach
Sequencing apparatus Martin D. Leach
Biotech Company WWW registry Martin D. Leach
Request for actin clone (?) Martin D. Leach
RT-PCR using S1 Nuclease Martin D. Leach
CREB antibodies Martin D. Leach
ATCC e-mail Martin D. Leach
GENE EXPRESSION Martin D. Leach
PROMEGA ??? Martin D. Leach
Transfections using QIAGEN Martin D. Leach
Attachment of primary cell cultures Martin D. Leach
promoter/intron-exon people: wanna help me?? Martin D. Leach
Toothpick sources in the UK Martin D. Leach
core mills corp Martin D. Leach
Suggestions for increasing phage stability? Kevin Lease
why fix sequencing gel? K. Legate
Seeking ß-Gal plasmid with nuclear targeting signal! Sigrid Lehnert
Methylmercuric hydroxide- SOURCE? Nancy Lemieux
What's wrong with my DNA sequencing template? Michel Leroux
PCR sequencing problem Walt Lilly
DNA Fingerprinting - Simpson Trial George Lin
pGex-cryptic promoters in GST? Dale Lindsley
silver sequencing Eric W. Linton
Silver Sequencing Eric W. Linton
Univ. charging $100/mo./person for mainframe access Lisa
Random Peptide Libraries Workshop R. Wayne Litaker
Re-using hybridisation solutions containing double-stranded probes? Jim Litts
EtOH precipitation Jim Litts
Northern with non-radioactively labelled probe Muhammad Lodhi
ligation/transformation: HELP ME PLEASE!! Steven Loechel
pSV3-neo Steven Loechel
neuronal cell line (guinea pig) Thomas Loeffler
Anti P-Ser/P-Thr Antibodies Lani Louise Longenecker
pLysS freezer stock instability Frank van de Loo
Measurement of cholesterol in mice Lopez-Molina Luis
Cholesterol-rich food for mice Lopez-Molina Luis
Plasmid Maps R. John Lye
Sequencing apparatus R. John Lye
VectorNTI R. John Lye
ligation/transformation: HELP ME PLEASE!! Alan Lyons
Pig cDNA libraries: Testis, Ovary/oocyte or brain Ian Lyons
Electrophoresis Gel? Ian Lyons
DNA integration after transfection of mammalian cells Ian Lyons
EtBr in agarose gels... Ian Lyons
protein/polysach contaminants M.DELAVINA at CGNET.COM
Fluorescein-11-dUTP M.P.Woodland
peptide expression in E.coli M.R.douglas at rheuma.bham.ac.uk
High mol wt bFGF bands-repost N.Ambalavanan MD
Plasmid nomenclature MUNGO at UVVM.UVIC.CA
transfection of primary cells NEAL MUSTO
transfection of plasmids into E. Coli NEAL MUSTO
BNN132 strain Colin MacD
Thiofusion expression system Emma Macfarlane
Problems with densitometry!....for WESTERN BLOTS Gerard J Madlambayan
SV40 Giovanni Maga
SV40 (fwd) Giovanni Maga
Proteinase K Mail at scri.sari.ac.uk
TA cloning -Promega Mail at scri.sari.ac.uk
X-ray film storing Rafael Maldonado
E.coli "gene knockout": help Rafael Maldonado
Killer gene Rafael Maldonado
Pure bacterial chromosomal DNA Rafael Maldonado
Two hybrid Q. Rafael Maldonado
Freezing Bacteria-Liquid N2 OK? Rafael Maldonado
Direct selection for GalE in E.coli Rafael Maldonado
Curing E.coli plasmids Rafael Maldonado
shake after electroporation? Rafael Maldonado
LacZ Xsomal deletions Rafael Maldonado
Carboxy-terminal Antibody to gp120 Richard Mandel
Antibodies against Thioredoxin? Richard Mandel
test Helen Manley
Plant DNA Isolation John Marcus
Graphing Programme John Marcus
Graphing Programme John Marcus
Staining nitrocellulose Ferenc Marincs
Can you "fix" agarose gels? Ferenc Marincs
How do I make a TA cloning vector? Markus Grompe, MD
Repeat PCR for mouse genomic DNA Markus Grompe, MD
RT-PCR Monica J. Martick
GAL4 Antibodies? Dr. S.L. Mathias
SYBR green Mauro
DHFR substrates that can be crosslinked? Mark N. Mayhew
Metabolic pathways poster? Tom Maynard
address of Molecular Probes Inc. ?? Tom Maynard
Phosphatase Columns Andrew G. McArthur
Smiling sequencing gels Daniel Mcgoldrick
Invitrogen SP6 and T7 primers... E. J. Meharra
Ab for ferritin? Ojar Melefors
Desferrioxamine and E.coli. Ojar Melefors
in vitro transcription/ab-depletion Ojar Melefors
Ligation of alkaline phosphatase treated DNA David Micklem
Church hyb solution questions David Micklem
Q about boiling miniprep sequencing. David Micklem
help and advice on 5' RACE Mikek
Protein-DNA Crosslinks Chuck Miller
Univ. charging $100/mo./person for mainframe access Richard H. Miller
pET expression system Steve Minchin
thrombin antibodies? Ding Ming
GCG manual Ding Ming
Preparartion of Liver DNA for PFGE Dr Saleem Mohammed
Formaldehyde RNA gels. Dr Saleem Mohammed
genomic dna Mr Vid Mohan-Ram
cdna amplification Mr Vid Mohan-Ram
cdna pcr Mr Vid Mohan-Ram
HELP: Microtubuli or tubulin Thomas Mohr
help and advice on 5' RACE Robert Molday
who sells cheap MOPS. PLEASE.****** Sean David Moore
random hexamers as primers for RT in RT-PCR Sean David Moore
MULV-RT Sean David Moore
Gel box less than 1 dollar Sean David Moore
FOR SALE: Discount Lab Glassware Sean David Moore
wierd cell culture contam Sean David Moore
CsCl vs column kits Sean David Moore
Need human B cell expression library Carlos Moreno
UK oriented lab digest (Diagnosis) on WWW - ng-info.txt [1/1] Pam Moreton
Issue 1 of new U.K oriented DIAGNOSIS magazine Roger Moreton
UK oriented lab digest (Diagnosis) on WWW - ng-info.txt [1/1] Roger Moreton
Molar extinction coefficient of a protein at 280 nm. Ralf Morgenstern
E. coli W3110 not accepting two plasmids Ralf Morgenstern
primer for RT-PCR Shahram Mori
EtBr in agarose gels... Shahram Mori
Question: PCR With 500bp Primer? Shahram Mori
who sells cheap MOPS. PLEASE.****** Shahram Mori
Drosophila actin probe for RNase protection wanted Shahram Mori
Enhancement of PCR? Shahram Mori
primers in RT in situ PCR Shahram Mori
who sells cheap MOPS. PLEASE.****** Shahram Mori
random hexamers as primers for RT in RT-PCR Shahram Mori
GST-Fusion Shahram Mori
la pcr complexes Shahram Mori
Rf: How "random" are random primers? Shahram Mori
Phenol distillation Shahram Mori
Want PCR to incorporate 32P label Shahram Mori
can formamide harbour RNases? Shahram Mori
Need Input on Northern With Bacterial RNA Shahram Mori
Stratagene SurfZAP phage display vector Shahram Mori
PCRs no longer working. HELP! Shahram Mori
antibody compatibility Shahram Mori
RT-PCR using S1 Nuclease Shahram Mori
RT-PCR using S1 Nuclease Shahram Mori
PROMEGA ??? Mike Morris
Mutagenesis : help! Mike Morris
ligation/transformation: HELP ME PLEASE!! Heidi Moss
UV damage, dephosphoryl., and making constructs: MAYBE it's SOLVED??? Heidi Moss
HELP ME!! (lig/trans: UV and CIP) -- repeat message Heidi Moss
promoter/intron-exon people: wanna help me?? Heidi Moss
Does Klenow proofreading multiple-base 3' overhangs or just single base? Pete Muriana
How long would it take to clone a gene from a plasmid? Pete Muriana
How long would it take to clone a gene from a plasmid? Pete Muriana
COS-1 cells wanted Bernard Murray
Two hybrid Q. Bernard Murray
Riboflavin in Acrylamide Tony Murray
Rhodamine-4-dUTP and biotin-11-dUTP Tony Murray
On/Off rates of Rec. Fabs? Mxm
recombinant protein purification Mxm
Periplasmic extract/His tagged Prot?? Mxm
CsCl vs column kits Peter Myler
ELECTROPORATION PARAMETERS FOR LETTUCE PROTOPLASTS NICOL,NATHALIE,MS
Quantitative PCR Rajesh R. Naik
Marathon-RACE (clontech) inquiry Jon Nakamoto
Ligation problems Francois Nantel
Chromosome seperation Frank Narz
DNA elution from gels Dr. Kent L. Nastiuk
Precut nylon hybridization membranes? Dr. Kent L. Nastiuk
Want PCR to incorporate 32P label Dr. Kent L. Nastiuk
PCR with few target on spinal fluid Dick Nelson
Protein-DNA Crosslinks Scott A. Ness
RefMan Rick Neubig
RefMan Rick Neubig
Insect Phenoloxidase Lisa Neven
Disposable filters for buffers etc John A. Newitt
NIH Phone Number John A. Newitt
Running Retic lysate on SDS-PAGE John A. Newitt
farnesyl transferase John A. Newitt
Slowly migrating EGF receptor in SDS-PAGE John A. Newitt
Slowly migrating EGF receptor in SDS-PAGE John A. Newitt
ECL problems John A. Newitt
E.coli "gene knockout": help Brad Nicholson
Suggestions for increasing phage stability? Brad Nicholson
GENE EXPRESSION Soeren Jensby Nielsen
(none) To: Nikolai
MW of IgG? To: Nikolai
Cross-linking in proteins? To: Nikolai
EtBr in agarose gels vs. EtBr staining later Rae Nishi
RT-PCR of Rare mRNA Rae Nishi
(none) Gustavo Nolasco
(none) Gustavo Nolasco
mRNA in situ on sections with DIG-probe? Katarina Nordqvist
Does Klenow proofreading multiple-base 3' overhangs or just single base? Pamela Norton
RNAse protection assay Pamela Norton
Optimum temp for PCR primer annealing Andy Law Big Nose
Chelex protocol? Karen B. O'Brien
n Karen B. O'Brien
direct sequence analysis of PCR products Kevin O'Connell
Methylmercuric hydroxide- SOURCE? Thomas O'Neill
DHFR and methotrexate Thomas O'Neill
Methylmercuric hydroxide- SOURCE? Thomas O'Neill
MICROSATELLITE ISOLATION Philip OBrien
size determination of rflps Ola
TRANSFER OF PLASMIDS INTO BACTERIA Kevin Oldenburg
TA cloning kit (In Vitrogen) Onno_Myriam
TA cloning kit (In Vitrogen) Onno_Myriam
anti-mouse integrin/neutrophil mAb Douglas Ormrod
HELP ME!! (lig/trans: UV and CIP) -- repeat message -Reply David Osterbur
CsCl preps for ABI sequencing reactions Anthony Otsuka
ATCC Catalogs? Francis Ouellette
pWE15 Cosmid vector Francis Ouellette
Phenol distillation Jim Owens
DNase treatment of RNA sample Jim Owens
PCR primer design Jim Owens
[Q]Antibody database Koichi Ozaki
RAPD with Air Thermal Cycler UDAY PATHRE
Lambda phage mimotopes library PAULINO GOMEZ PUERTAS. CISA-INIA. VALDEOLMOS. 28130, MADRID.
Source of SceI Javier PEREA
GFP PJOB
Kinase Substrate Sequences? PJOB
address of Molecular Probes Inc. ?? BANANA & PLANTAIN
problems with yeast two hybrid system ANDREI POPOV
hIgG1 plasmid ANDREI POPOV
ELISA for human IgG ANDREI POPOV
Source of SceI ANDREI POPOV
RNA from spleen ANDREI POPOV
Urea SDS-PAGE ANDREI POPOV
Any one used Oligos from Universal DNA Inc? $1/base! PRAKASH at ACD.TUSK.EDU
GFP review PRASHERD at DELPHI.COM
Pischia expression PWHoward
taq preparation Prasit Palittapongarnpim
Direct sequencing of PCR products Christopher Paul Palmer
Pfu/Taq in PCR reactions Christopher Paul Palmer
Cloning using lamda-phage TA cloning kit Andrew Parolin
Freezing Bacteria-Liquid N2 OK? Aaron Pawlyk
RNA from Spleen - Help! Aaron Pawlyk
RNA from Spleen - Help! Aaron Pawlyk
Help! RNA extraction Aaron Pawlyk
RT-PCR using S1 Nuclease Petra M. Jakobs, Ph.D.
Why can't I get agarose embedded HMW DNA to digest ? Howard T. Petrie
Buying a thermocycler Gregory E. Gonye PhD
who sells cheap MOPS. PLEASE.****** Stephen P. Driska PhD
Oligo 4.0-s program Pierre_Talbot at Infopuq.UQUEBEC.CA
PCR primer design Pierre_Talbot at Infopuq.UQUEBEC.CA
Problems with densitometry!....for WESTERN BLOTS T.S. Pillay
Problems with densitometry!....for WESTERN BLOTS T.S. Pillay
cDNA library construction A van der Plas
TA Cloning and screening Mike Poidinger
MEDLINE. Mike Poidinger
Whatman Filter Paper Aaron Polesky
How "random" are random primers? Richard T. Pon
Bacteriophage Strain Collection Reno Pontarollo
PCR trouble with Arabidopsis gene Bert Popping
Neomycin sulfate and G418 Mike Preston
EtBr in agarose gels... Robert Preston
Toothpicks for PCR Robert Preston
Getting out the brown from DNA Robert Preston
PCR Inhibition Water Chloramines Robert Preston
Culturing tumors in 3D Pieter Proot
3D tumor culturing Pieter Proot
Definition: Saturation Mutagenesis Rock Pulak
Transfections using QIAGEN QIAGEN
Qiagen 96-well QIAGEN
German Merck contact Jennifer C. Quirk
EtBr decontamination NATHALIE RANCIAT
Pischia expression RCT2BNC
Pischia expression RCT2BNC
Help needed-- understanding POROS / Perfusion chromatography RMARTINQFD at BIOTECHNET.COM
extraction of DNA from blood clots MIRIAM P. ROSIN
GFP ROSS at SCI.WFEB.EDU
COS-1 cells wanted Uttam Rajbhandary
Multiple IgG bands in reduced SDS-PAGE Dayanidhi Raman
Slowly migrating EGF receptor in SDS-PAGE Dayanidhi Raman
Need Human Stromal cDNA library Nagarajan Ramesh
Electrophoresis Gel? RandBio
Wax beads RandBio
QIAquick Spin Columns Pekka Rappu
Killer gene Pekka Rappu
Killer gene again Pekka Rappu
200 microliter m'fuge tube storage racks Jeff Rawlings
Total cellular protein vs cell number Jeff Rawlings
protein kinase A inhibitors Jeff Rawlings
Problems with densitometry!....for WESTERN BLOTS Jeff Rawlings
cytokine Response elements Alec Redwood
green fluorescent proteins Alec Redwood
Algae DNA Alec Redwood
RNA from Spleen - Help! Alec Redwood
core mills corp Alec Redwood
help and advice on 5' RACE Robin Reed
need help on RNA isolation from plant Sharon Regan
P1 library Screening Marko Rehn
pWE15 screen Tanya Reichert
Sequencing Gels Reid, Thomas M.
Hybridization Protection Assay Udo Reischl
Software for interpruting restricton digests. Larry Reiter
Sequencing Lambda DNA Rene.DeMot at agr.kuleuven.ac.be
Electrophoresis Help Essex Research
E.coli "gene knockout": help Richard_Heath
Restriction Digestion and Bacteria Richard_Heath
pLysS freezer stock instability Richard_Heath
subscribe Christopher Thomas Richie
Linear Polyacrylamide (Ian Ridgers)
DNA FROM WHOLE BLOOD (Ian Ridgers)
BbvII Restriction enzyme Paul Riggs
suppliers of Fse1,Sse83871, BspG1 ??? Paul Riggs
UV Crosslinking Protein/DNA Linda M Ritter
pHC79, cosmid vector; supply Robert.Coelen at jcu.edu.au
(none) Leonora Rochwerger
mRNA half-life Leonora Rochwerger
little blue homogenisers David R. Rockhold
Drosophila actin probe for RNase protection wanted Richard M. Rohan
DNA oligomers on acrylamide vs. agarose gel Nina Rosario L. Rojas
Ru 486 Season Rose
high background slot blots Mike Rott
Yeast plasmids sought Peter J. Russell
OXYGENE DALLAS? Doug-young Ryu
NIH Phone Number Doug-young Ryu
PCR enz:Taq/Pwo Doug-young Ryu
CLONING PCR PRODUCT Doug-young Ryu
GCG manual Doug-young Ryu
potato tuber RNA? Gellatly Kevin S
Subcloning large fragments S.
apoptosis detection: problems with test kits SHO1
apoptosis detection method SHO1
control for TPA induction Manuel Maria SIMON
Church for Northern blots? Manuel Maria SIMON
CO2 Cell Culture Incubator Manuel Maria SIMON
Want PCR to incorporate 32P label Manuel Maria SIMON
CAT assay problem! Manuel Maria SIMON
VectorNTI Manuel Maria SIMON
SPR independent translocation into ER Manuel Maria SIMON
in vitro transcription/translation Manuel Maria SIMON
phosphoimager Manuel Maria SIMON
Temp. Gradient Gel Electrophoresis/GC clamps MARLENE SNYDER
Oligo 4.0-s program SPAGNOL at PLAUTO.CSATA.IT
An IPCR Tale SPAGNOL at PLAUTO.CSATA.IT
Proteinase K SPAGNOL at PLAUTO.CSATA.IT
Amplicon's purification. SPAGNOL at PLAUTO.CSATA.IT
Taq-Pfu compatibility SPAGNOL at PLAUTO.CSATA.IT
BAC library construction, I need help! SYME,JENNIFER ALBERTA,MS
BAC, cloning, HELP! SYME,JENNIFER ALBERTA,MS
ligation/transformation: HELP ME PLEASE!! Klaus Salger
Proteinase K Klaus Salger
"missing" RNA Klaus Salger
Ligation problems Klaus Salger
Exo-Meth Sequencing Klaus Salger
Ligation problems Klaus Salger
CAT assay problem! Klaus Salger
GAL4 Antibodies? Konstantine Sarafis
Retrieval of ssDNA from a mixture of ssDNA/dsDNA Mr. J. Sasse
An enzyme degrading dsDNA but not ssDNA Mr. J. Sasse
DNA prep for transient transfection Ken Saville
Electrophoresis Gel? Ken Saville
Drosophila FM6 balancer problem Ken Saville
RNAse protection assay Katherine Schaefer
RNAse protection assay FIXED! Katherine Schaefer
recombinant protein purification Dominique Schaller
recombinant protein purification Dominique Schaller
Wanted-Used Equipment Richard Schifreen
blue band Denni Schnapp
PS blue band Denni Schnapp
Qwik-precip Ann Schofield
Phenol distillation Edgar Schreiber
Rubisco clone Alan Schulman
M13 antibodies for phage display Schuster, David
DHFR and methotrexate Barry Schweitzer
why fix sequencing gel? Daniela Sciaky
sequence programs Daniela Sciaky
Staining nitrocellulose Bart Sefton
CLONING PCR PRODUCT Loryn Sellner
Wax beads G Shafer
Proposed FDA Antibody Regs May Affect You G Shafer
hIgG1 plasmid availability Gwen Shafer
Large Scale Insect Cell Culture Gwen Shafer
WHO SELLS EXO-KLENOW??? Sunil Shaw
Church hyb solution questions Glen Shearer
Church hyb solution questions Glen Shearer
Re-using hybridisation solutions containing double-stranded probes? Glen Shearer
DMSO in PCR rxns ?? Glen Shearer
Church for Northern blots? Glen Shearer
Church hyb solution questions Glen Shearer
Church for Northern blots? Glen Shearer
EtBr , How do you get rid of it? Greg Shen
cloning with PCR Sheris at OSUUNX.UCC.OKSTATE.EDU
digital gel documentation Michael A. Shia
need Ab for poly (ADP-ribose)polymerase Incheol Shin
DNA Fingerprinting - Simpson Trial Richard E. Showalter
DNA Fingerprinting - Simpson Trial Richard E. Showalter
M-13 Purification kits Richard E. Showalter
PCR primer design Richard E. Showalter
REVISED SOFTWARE: Random Cladistics 2.1.1 Mark Siddall
SYBR green Sandy Silverman
pcDNA3 Chris Silvia
Nonidet P-40 Chris Silvia
GST-Fusion L Bruce Sk
GeneClean vs Eluquik vs Prep-a-gene Skory
BbvII Restriction enzyme Rick Smith
WHO SELLS EXO-KLENOW??? Rick Smith
Can you "fix" agarose gels? Rod Snowdon
Procaryotic RNA isolation Daniel Solaiman
Chelex Sophie Launey, These Andre Gerard 31 12 96, Ifremer DRV RA, 46.36.30.07
Triton X-114 Endotoxin Removal-S.O.S. Martin Sorich
Triton X-114 Endotoxin Removal-S.O.S. 2 Martin Sorich
human polyA DNA Anne Spang
Precut nylon hybridization membranes? Silvia Spitzer
Kits for genomic DNA from blood (lymphocytes) Silvia Spitzer
WHO SELLS EXO-KLENOW??? Darren M. Stauth
GST fusions Rob Steele
Mobility Shift and Glutamic Acid Stefan Einar Stefansson
Non-radioactive cDNA library screening Bernd Stein
5' and 3' end of cDNAs by PCR Bernd Stein
HELP: streaks in isozyme electrophoresis Mikael Stenstrom
Autoclaving Dextran Sulfate? Stephen R. Lasky, Ph.D.
Alternatives to Formalin/Formaldehyde? Stephen R. Lasky, Ph.D.
degenerate primers PCR David B. Stern
used lab equipment Neal Stewart
Rhodococcus Phage Q4 Matthew Stoecker
Inverse PCR on cDNA Gordon Stott
NEEDED: micro-solid phase extraction column Michael A. Strausbauch
Microwave techniques? Steven Striepeke
methicillin&ampicillin Zal Suldan
Enhancement of PCR? Zal Suldan
NIH Phone Number Zal Suldan
Want PCR to incorporate 32P label Zal Suldan
Elution from Glutath. beads Zal Suldan
Competent Yeast Cells Zal Suldan
primer for RT-PCR Steven Sullivan
RT-PCR of Rare mRNA Steven Sullivan
Question: PCR With 500bp Primer? Steven Sullivan
Question: What does BSA do in PCR reactions? Steven Sullivan
Long PCR hints -- any sources? Steven Sullivan
Formaldehyde RNA gels. Steven Sullivan
tgf-beta Clemens Suter-Crazzolara
IN SITU RT-PCR Agnieszka Szczepek
Autoclave to remove RNAses. TUCKER at AFP76.HUMC.EDU
DNase inhibitor S. Takeshita
Any pACYC plamids with T7 promoters? Scott Taylor
Elution from Glutath. beads Dennis Templeton
The One-dollar Gel Box-Graphite plates for blotting Dennis Templeton
CO2 Cell Culture Incubator Anton Tevelev
Methylmercuric hydroxide- SOURCE? Tom Thatcher
Dyes and supercoiled DNA Brad Thomas
Kits for genomic DNA from blood (lymphocytes) Brad Thomas
CO2 Cell Culture Incubator Brad Thomas
Rapid Polyacrylamide Polymerization Brad Thomas
DTT in in vitro transcription Rx Matt Thomas
eosophageal cells Alan Thornley
Inducible mammalian expression vectors mammalian Alan Thornley
Electrophoresis Gel? David John Thornley
A.S.A.P GENOMIC DNA ISOLATION KIT Ketil Thorstensen
Epitope tagged molecules Richard T. Timmer
Church hyb solution questions Anthony Tomlinson
How many DNA binds to Nylon membranes Anthony Tomlinson
Ligation problems Anthony Tomlinson
SDS Removal Houthaeve Tony
Exo-Meth Sequencing Janne Tornow
Advice on mitochondria isolation from fungi (i.e. percoll gradient). Gus Trautweiler
MS-DOS program for fragment sizing from digitisers Martin Trick
plasmid isolation from yeast Dirk Truman
Competent Yeast Cells Dirk Truman
Phosphorimager Questions Mark W. Trumbore
Disposable filters for buffers etc Mark W. Trumbore
EtBr in agarose gels vs. EtBr staining later John Tudor
Marathon-RACE (clontech) inquiry Bradley Turner
Marathon-RACE (clontech) inquiry Bradley Turner
Methylmercuric hydroxide- SOURCE? Bradley Turner
address of Molecular Probes Inc. ?? Bradley Turner
EtBr , How do you get rid of it? U12201 at uicvm.uic.edu
alternative to silver stain? U12201 at uicvm.uic.edu
size determination of rflps U12201 at uicvm.uic.edu
Companies with E-mail/Gopher/WWW U12201 at uicvm.uic.edu
immunofluorescence background U12201 at uicvm.uic.edu
(none) U12201 at uicvm.uic.edu
Cholesterol-rich food for mice U12201 at uicvm.uic.edu
Silver Sequencing U12201 at uicvm.uic.edu
storing western blots U12201 at uicvm.uic.edu
Direct fluorescein labeling of DNA U12201 at uicvm.uic.edu
Journal Table of Contents U12201 at uicvm.uic.edu
PKC assay wanted!! U12201 at uicvm.uic.edu
Problems with densitometry!....for WESTERN BLOTS U12201 at uicvm.uic.edu
Chemical Cleavage of Proteins/Peptides U12201 at uicvm.uic.edu
(none) U12201 at uicvm.uic.edu
MW of IgG? U12201 at uicvm.uic.edu
Toothpick sources in the UK U12201 at uicvm.uic.edu
Nonidet P-40 U12201 at uicvm.uic.edu
Cross-linking in proteins? U12201 at uicvm.uic.edu
Nonidet P-40 U12201 at uicvm.uic.edu
DNA Fingerprinting - Simpson Trial U27111 at uicvm.uic.edu
Getting out the brown from DNA Paul E. Ulanch
HISTONS / who is (Your Name)? Daisuke Umeno
Help! RNA extraction Unknown
FAS(CD95) and FASL Jeffrey Upperman
Plasmid Diagrams Karen Usdin
question about P1 expression library User
Regulated expression cloning vectors HELP Edgar Valencia
y Rika Vanhuizen
y Rika Vanhuizen
y Rika Vanhuizen
Activated charcoal in DNA preparation ?? Lisa Vawter
HepG2 transfection w/liposomes Vennie
oxidation of SS bridges Vennie
GUS immunohistochemistry Stan Vitha
Help: I need the plasmid pGFP-N3 (distributed by Clontech) Werner Vogetseder
Help: I need a plasmid: pGFP-N3 (green fluorescent protein) Werner Vogetseder
Re-using hybridisation solutions containing double-stranded probes? Void
EtBr in agarose gels vs. EtBr staining later Karl Voss
Algae DNA WAS
Slow PhosphoImager Bernard Khoo WCI
Drosophila actin probe for RNase protection wanted Bernard Khoo WCI
One-step inactivation of RNase in Ambion RPA kit Bernard Khoo WCI
-----------------UK----------------------- PN WRIGHT
TA cloning -Promega Edward Wang
TA cloning ratio Edward Wang
PCR & spermidine Shiao Y. Wang
Graphing Programme Shiao Y. Wang
AMOUNT OF PRIMER IN DNA FINGERPRINTING Shiao Y. Wang
Autoclaving Dextran Sulfate? Shiao Y. Wang
Temperature gradient gel electrophoresis (TGGE) Shiao Y. Wang
Want PCR to incorporate 32P label Shiao Y. Wang
Proteinase K Shiao Y. Wang
0.2-mL PCR tubes Shiao Y. Wang
Need Input on Northern With Bacterial RNA Christine Ward
Plasmid Diagrams Pearse Ward
test Greg Warren
test Greg Warren
Nla IV - Restriction Enzyme Keith G. Weinstock
Univ. charging $100/mo./person for mainframe access Angela Werner
primers in RT in situ PCR Rob Wesley
TRANSFER OF PLASMIDS INTO BACTERIA David J. Westenberg
digital gel documentation Johnny Wharton
pre-cut culture dish membranes Deborah Lynn William
GPI anchors Iain Wilson
CO2 Cell Culture Incubator John Wolff
Phenol distillation John Wolff
Trouble with Fisher brand cartridge for Nanopure Donald Chun Kit Wong
PCR primer design Julian Wood
Anti P-Ser/P-Thr Antibodies Jim Woodgett
Kinase Substrate Sequences Jim Woodgett
Is it Taq or the primer responsible for the mistake? Robert Woodward
What's the most efficient way to remove DNA from polacyrlamide gels? John Wurzelmann
SKILLS REQUIRED BY CORPORATE LABS XYZZYX at DELPHI.COM
Charon 4a Yamamoto
RE map software Aurel Yang
software to generate restrcition fragment graph Aurel Yang
pfu polymerase Yi, Xiaoming
Q:Does Gene Clean interfere with ligation? Yi, Xiaoming
formaldehyde pH Harry Yim
alternative to silver stain? Christopher B. Yohn
digital gel documentation Christopher B. Yohn
Library rescue protocols Ian A. York
TRANSFER OF PLASMIDS INTO BACTERIA Ian A. York
DNA prep for transient transfection Ian A. York
pcDV1 - what is it? Ian A. York
Qiagen preps in transient transfections are OK Ian A. York
Transfection efficiency Ian A. York
pcDNA3 Ian A. York
CsCl vs column kits Ian A. York
Mam. Exp. Vector Masayuki Yoshida
E.coli "gene knockout": help Chris Yost
BSA Tom Young
VEGF ZHENGYI at HELIX.MGH.HARVARD.EDU
negative zymography of PAIs Luigi Zampieri
negative zymography Luigi Zampieri
AIDS Research Brent Zanke
DMSO in PCR rxns ?? Matthias Zeiner
DMSO in PCR rxns ?? Matthias Zeiner
MeOH in Western Buffer Matthias Zeiner
need hsp70 Matthias Zeiner
biotin-avadin in IP Xiangjun Zhou
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES Xin-Hua Zhou
Toothpicks for PCR Guan Zhu
DTT in in vitro transcription Rx Frances Hannan Zoology
Help: I need a plasmid: pGFP-N3 (green fluorescent protein) Frances Hannan Zoology
Pulse field gels MS WF HONG [GEN]53872
polystyrene biodegradation a
troponin I a.buchan
AD test posting a.dodd at auckland.ac.nz
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES a.dodd at auckland.ac.nz
Collegen assays a.vijayan
la pcr complexes aEscher
Will this experiment work? (p53-related) aEscher at ccmail.llu.edu
DNA sequence puzzle! aEscher at ccmail.llu.edu
RUBISCO ANTIBODIES agpcrv at palm.cri.nz
Cross-linking in proteins? ahillar at your-servername.Lan1.UManitoba.CA
Electroporation of RNA in cos-cells alechi at BIO.EMBNET.SE
Mosaicism in X. laevis embryos anmab2 at orion.alaska.edu
buying a thermocycler anne at lmb1.rug.ac.be
differential display anne at lmb1.rug.ac.be
TEST anonymous at somehost.somedomain
transfection quality DNA- help apeel at maple.circa.ufl.edu
CLONING PCR PRODUCT b-butler at nimr.mrc.ac.uk
Stable episomal vector in mouce cells bartd at lmb1.rug.ac.be
mouse vs human bcdlee at hkpucc.polyu.edu.hk
HYbridization Incubator bcdlee at hkpucc.polyu.edu.hk
suggestions on automated DNA sequencer? bcdlee at hkpucc.polyu.edu.hk
heteroplasmy of cloning vectors "Alexander Kraev" bckraev at aeolus.ethz.ch
Denaturing ds DNA for sequencing "Alexander Kraev" bckraev at aeolus.ethz.ch
Church hyb solution questions "Alexander Kraev" bckraev at aeolus.ethz.ch
Plaque PCR mystery "Alexander Kraev" bckraev at aeolus.ethz.ch
ligation/transformation: HELP ME PLEASE!! "Alexander Kraev" bckraev at aeolus.ethz.ch
Church hyb solution questions "Alexander Kraev" bckraev at aeolus.ethz.ch
Toothpicks for PCR "Alexander Kraev" bckraev at aeolus.ethz.ch
why fix sequencing gel? "Alexander Kraev" bckraev at aeolus.ethz.ch
Sequencing Gels "Alexander Kraev" bckraev at aeolus.ethz.ch
UV damage, dephosphoryl., and making constructs: MAYBE it's SOLVED??? "Alexander Kraev" bckraev at aeolus.ethz.ch
Q : E. coli instability "Alexander Kraev" bckraev at aeolus.ethz.ch
Ligation problems "Alexander Kraev" bckraev at aeolus.ethz.ch
M-13 Purification kits "Alexander Kraev" bckraev at aeolus.ethz.ch
Toothpicks for PCR "Alexander Kraev" bckraev at aeolus.ethz.ch
Ligation problems "Alexander Kraev" bckraev at aeolus.ethz.ch
TA cloning ratio "Alexander Kraev" bckraev at aeolus.ethz.ch
Ligation problems "Alexander Kraev" bckraev at aeolus.ethz.ch
CsCl preps for ABI sequencing reactions "Alexander Kraev" bckraev at aeolus.ethz.ch
PCRs no longer working. HELP! "Alexander Kraev" bckraev at aeolus.ethz.ch
Subcloning large fragments "Alexander Kraev" bckraev at aeolus.ethz.ch
Request for Site directed mutagenesis Protocols (PCR) benedik at uh.edu
Is it Taq or the primer responsible for the mistake? benedik at uh.edu
EtOH precipitation benedik at uh.edu
Denaturing ds DNA for sequencing benedik at uh.edu
Luminometer info? berickson at ntet.nctr.fda.gov
Electroporation of Antibodies gettin' better
G-C content determination paul betts
Pulse field gels biukovic at olimp.irb.hr
GENE CLEAN biukovic at olimp.irb.hr
Fructans and fructose? bjorn
Sequencing apparatus bjvv at scinet2.up.ac.za
pET expression system bllim at hkucc.hku.hk
Stratagene's Quik Hybridization solution books at mcvax2.d48.lilly.com
Does Klenow proofreading multiple-base 3' overhangs or just single base? brett at BORCIM.WUSTL.EDU
Ligation problems brett at BORCIM.WUSTL.EDU
Electroporation of Antibodies brett at BORCIM.WUSTL.EDU
Stain for Copper Proteins marius brouwer
Wanted - Murine IL-10 cDNA construct bwalker at comp.nibsc.ac.uk
Who sells "UNGRAPH" software? bwr at gnv.ifas.ufl.edu
Labelling proteins in plant protoplasts c515455 at mizzou1.missouri.edu
Why can't I get agarose embedded HMW DNA to digest ? cavell at BBSRC.AC.UK
AZTREONAM, HELP! chengs1 at jaguar.uofs.edu
Long PCR with Taq and Vent quin chou
TA cloning : clone contamination chukins
Fluorescent Proteins Newsgroup proposal clontech!paulki at NETCOM.COM
electroporator colleen at chempath.uct.ac.za
Attention Agnes Tay Hou Ngee contente at usuhsb.usuhs.mil
Mouse cDNA for EGFR croyleml at ucbeh.san.uc.edu
pGEX (GST) expression system bipin dalmia
Large Scale Insect Cell Culture bipin dalmia
protein expression on a contract basis bipin dalmia
WANTED: research associate in protein expression bipin dalmia
Adress of Tetko, Elmsford, NY derk at hubrecht.nl
Cracked SSCP gels r.j. dewar
Software for oligo dieter.deforce at rug.ac.be
PCRs no longer working. HELP! dieter.deforce at rug.ac.be
Metabolic pathways poster? dleviten at p001655.icos.com
Metabolic pathways poster? dleviten at p001655.icos.com
SIB acronym dtk
Xenopus liver cDNA library elder at CGL.UCSF.EDU
PCR using lambda gt11 primers elida at parasit.lan.mcgill.ca
Bacteria cell lysis rogers elizabeth
CAT assays in E. coli rogers elizabeth
CAT assays in E. coli rogers elizabeth
Km of glutamate dehydrogenase enrcaslt at eucmvx.sim.ucm.es
Transfection of human primary fibroblasts eric at ericpc.mni.mcgill.ca
VectorNTI frits at hubrecht.nl
Agarose Gel Loading Dye hAPPY gUY
Total cellular protein vs cell number ggza at umich.edu
polyclonal anti-mouse-hsp90 wanted ghilardi at ubaclu.unibas.ch
READ/NEW/FOLLOWUP gough_k at wehi.edu.au
methicillin&ampicillin kevin c. graham
shake after electroporation? kevin c. graham
pSG5 form Stratagene! gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
Help on PCR using modified DNA bases guru at STUBBE-LAB.MIT.EDU
German Merck contact gustilo at pobox.upenn.edu
Operational Amplifiers gvacano at eagle.wesleyan.edu
Bio-Rad Phosphorimager h
Running Retic lysate on SDS-PAGE h
Direct fluorescein labeling of DNA hanzel at mdyn.com
in vitro transcription/ab-depletion hcavaco at fm.ul.pt
dig-RNA synthesis hcorbett
Methylmercuric hydroxide- SOURCE? rosemary hoffman
Who sells microsatellite primer/ probe kit? huangbx at DEAKIN.EDU.AU
MULTIPLE SCLEROSIS CONFERENCE irc at III.NET
DHFR and methotrexate jeg at PW.USDA.GOV
Testing... Do not read. jerian
Re-using hybridisation solutions containing double-stranded probes? jfh
PCR using lambda gt11 primers jfh
mouse vs human jfh
Problems with densitometry!....for WESTERN BLOTS jfs at WH.BAYER.COM
Sequencing Lambda DNA jitu
RT-PCR with Dynabeads jm
buying a thermocycler jm
Molecular Biology Short Course?? joanna at WH.BAYER.COM
Temperature gradient gel electrophoresis (TGGE) r jones
CAT assay problem! jr at dna.bio.warwick.ac.uk
RNA isolation kits (G+) jtbrown at osuunx.ucc.okstate.edu
GFP kang at msvax.mssm.edu
Question: What does BSA do in PCR reactions? kang at msvax.mssm.edu
Church hyb solution questions kang at msvax.mssm.edu
PCR & spermidine kang at msvax.mssm.edu
MAKING YOUR OWN GEL BOXES kang at msvax.mssm.edu
Highly competent cells kang at msvax.mssm.edu
Request for Site directed mutagenesis Protocols (PCR) kang at msvax.mssm.edu
DNA integration after transfection of mammalian cells kang at msvax.mssm.edu
Transgenic mice; is it easy or difficult? kang at msvax.mssm.edu
One step transformation kang at msvax.mssm.edu
Ligation of alkaline phosphatase treated DNA kang at msvax.mssm.edu
why fix sequencing gel? kang at msvax.mssm.edu
Gel box less than 1 dollar kang at msvax.mssm.edu
Want PCR to incorporate 32P label kang at msvax.mssm.edu
Q about boiling miniprep sequencing. kang at msvax.mssm.edu
methicillin&ampicillinREGISTER kitchingman at mbcf.stjude.org
Returned mail: research in flavors anantha r kolleivgode
UNSUBSCRIBE anantha r kolleivgode
serum and HeLa kreuzer at bkfug.kfunigraz.ac.at
P1 phage query hodges bradley l
How do I make a TA cloning vector? hodges bradley l
Pfu/Taq in PCR reactions hodges bradley l
PCRs no longer working. HELP! hodges bradley l
PKC assay wanted!! leon at hubrecht.nl
in situ, ethanol or methanol for dehydration? jie li
DMSO in PCR rxns ?? jie li
DTT in in vitro transcription Rx jie li
Amino Acid composition? lmjakt at hkucc.hku.hk
Protocol for downward blot lmjakt at hkucc.hku.hk
ornithine decarboxylaseinhibitor louis at lmb1.rug.ac.be
JBC journal (3.3.95) marcvdc at lmb1.rug.ac.be
ALTERNATIVE SPLICING marcvdc at lmb1.rug.ac.be
Qiagen 96-well maria at acme.llnl.gov
96-well microtiter plates maria at acme.llnl.gov
Nla IV - Restriction Enzyme stephen marshalko
Univ. charging $100/mo./person for mainframe access mathog at seqaxp.bio.caltech.edu
Conservation of extracellulaire matrix mazella at naxos.unice.fr
Antibodies against Thioredoxin? mccallo at randb.pprd.abbott.com
How "random" are random primers? mdcabl at cc.newcastle.edu.au
HELP: pyrroletricarboxylic acid, thiazoledicarboxylic acid michael.micksche at univie.ac.atBettinaKammerer
Optimum temp for PCR primer annealing mmg6
Temp. Gradient Gel Electrophoresis/GC clamps molpath at chmeds.ac.nz
Vent DNA polymerase and assymmetric PCR molpath at chmeds.ac.nz
Killer gene mpage at molbiol.ox.ac.uk
Southern with small probe? mpage at molbiol.ox.ac.uk
Getting out the brown from DNA mpage at molbiol.ox.ac.uk
Stable episomal vector in mouce cells mpk6
DNA prep for transient transfection mrb at WH.BAYER.COM
lyophilization of antisera nandid at ucbeh.san.uc.edu
Phosphorimager Questions neale at mbcf.stjude.org
GFP in CHO cells Grtful ned
GFP problems. Any tips? Grtful ned
FAS(CD95) and FASL Grtful ned
DNA prep for transient transfection nick at lmb1.rug.ac.be
software to generate restrcition fragment graph nico at lmb1.rug.ac.be
GST-Fusion ossipow
Elution from Glutath. beads ossipow
Exo-Meth Sequencing otunnela at ktl.fi
A beginner in RACE PCR, or possibly inverse PCR needs advice! paiks at gunet.georgetown.edu
promoter/intron-exon people: wanna help me?? paiks at gunet.georgetown.edu
Amino Acid composition? pathology at mscf.med.upenn.edu
Is it Taq or the primer responsible for the mistake? carlos h. pedemonte
Toothpick sources in the UK daniel petersen
Pfu/Taq in PCR reactions pgegen at rnaworld.bio.ukans.edu
unsubscribe picatags at TCPLINK.NREL.GOV
oligonucleotides DJC poland
alignment software DJC poland
antisense oligonucleotides DJC poland
Request for actin clone (?) pzhang
In vitro transcription ...... r3211001
s.moore
ligation/transformation: HELP ME PLEASE!! saboteur at BORCIM.WUSTL.EDU
Radiolabelled Novobiocin sbaker at molbiol.ox.ac.uk
DNA prep for transient transfection seppen at amc.uva.nl
COS-1 cells wanted seppen at amc.uva.nl
AB1899 phenotype shua!
Expression library for MHC H-2Db skcbiol
magnetic bead question sl1nn at cc.usu.edu
What is the expression vector, pSG5? slee at opal.tufts.edu
Stratalinker : help needed stephan at lmb1.rug.ac.be
Again! help for crosslinking proteins to DNA ? stephan at lmb1.rug.ac.be
test cynthia stryker
test cynthia stryker
test cynthia stryker
test, ignore cynthia stryker
CsCl vs column kits cynthia stryker
Need antibodies for ompA protein taylor
p53 gene theod at mel.dbe.csiro.au
p53 gene theod at mel.dbe.csiro.au
p53 gene theod at mel.dbe.csiro.au
GFP-Vectors with oriP ? thoenes
GFP: What is Coelenterazine thoenes
Electrophoresis Gel? tivol at tethys.ph.albany.edu
Univ. charging $100/mo./person for mainframe access tivol at tethys.ph.albany.edu
TA cloning : clone contamination txpljfg at uabcvsr.seas.ucla.edu
CMC of Octyl-POE? Moritz v.Rechenberg
Blot pictures vitor warwar
M13 antibodies for phage display williams at bscr.uga.edu
Swansoine willingmann at uke.uni-hamburg.de
Ligation problems wmbonner at helix.nih.gov
Two-Hybrid and Clontech wvcrieki at lmb1.rug.ac.be
2-D Electrophoresis ya-ya
Help for ApG !! yhping at ccvax
RNA electrophoresis ynakao at kean.ucs.mun.ca
IGFBP-3 ynakao at kean.ucs.mun.ca
UV crosslinking ynakao at kean.ucs.mun.ca
metal grid for raft culture ynakao at kean.ucs.mun.ca
RNA secondary structure programs your_mcmail_id
GST-Fusion zac
Differnetial Display zinetti at acpub.duke.edu

Last message date: Fri Mar 31 23:41:52 EST 1995
Archived on: Sun Mar 6 20:29:57 EST 2005

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