Methods March 1995 Archives by date

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Starting: Wed Mar 1 01:46:10 EST 1995
Ending: Fri Mar 31 23:41:52 EST 1995
Messages: 1461

Pig cDNA libraries: Testis, Ovary/oocyte or brain Ian Lyons
sub. of plant tissue culture newsgroup LEES at SASK.USASK.CA
GFP kang at msvax.mssm.edu
Question: What does BSA do in PCR reactions? kang at msvax.mssm.edu
Pulse field gels MS WF HONG [GEN]53872
mol genet forensic "bible"? Andre Hamel
Electrophoresis Gel? Ian Lyons
Metabolic pathways poster? (Dave Johnston)
negative zymography of PAIs Luigi Zampieri
Rf: Metabolic pathways poster? LOGAND
GFP ROSS at SCI.WFEB.EDU
EtBr in agarose gels vs. EtBr staining later John Tudor
Mam. Exp. Vector Masayuki Yoshida
Can you "fix" agarose gels? Rod Snowdon
Info on Santa Cruz Biotechnology Giwamot at indycms.iupui.edu
ligation/transformation: HELP ME PLEASE!! Heidi Moss
Riboflavin in Acrylamide Scott Selby Med Heme
Church hyb solution questions Glen Shearer
blue band Denni Schnapp
Direct sequencing of PCR products Christopher Paul Palmer
PS blue band Denni Schnapp
Culturing tumors in 3D Pieter Proot
Metabolic pathways poster? Martin D. Leach
Transfecting Primary Cell Lines Martin D. Leach
Church hyb solution questions kang at msvax.mssm.edu
question about P1 expression library User
Riboflavin in Acrylamide Tony Murray
transfection of primary cells NEAL MUSTO
Two-Hybrid and Clontech wvcrieki at lmb1.rug.ac.be
Neomycin sulfate and G418 Mike Preston
P1 phage query hodges bradley l
DNase inhibitor S. Takeshita
To Dave Gilbert (sorry, others may ignore this) Tracy Aquilla
ligation/transformation: HELP ME PLEASE!! saboteur at BORCIM.WUSTL.EDU
DNA elution from gels Dr. Kent L. Nastiuk
Anti P-Ser/P-Thr Antibodies Lani Louise Longenecker
Need mice in Dallas, TX area GORDON BETTS
MAKING YOUR OWN GEL BOXES Daniel Kim
GAP genomic sequences? C.J. Hussussian, MD
Univ. charging $100/mo./person for mainframe access Donald A. Lehn, Ph.D.
Re-using hybridisation solutions containing double-stranded probes? jfh
Transfecting Primary Cell Lines Dermot Cox
2-Mercaptoethanol, thanks Paul N Hengen
Riboflavin in Acrylamide Klaus.Matthaei at ANU.EDU.AU
Lambda phage mimotopes library PAULINO GOMEZ PUERTAS. CISA-INIA. VALDEOLMOS. 28130, MADRID.
VEGF ZHENGYI at HELIX.MGH.HARVARD.EDU
GFP PJOB
small microfuges/quick spins? Andre Hamel
Staining nitrocellulose Bart Sefton
GFP Dave Bates
Activated charcoal in DNA preparation ?? Lisa Vawter
Problems with yeast two-hybrid system Brian M. Cooke
Need Human Stromal cDNA library Nagarajan Ramesh
Plant DNA Isolation John Marcus
Graphing Programme John Marcus
Graphing Programme John Marcus
GUS immunohistochemistry Stan Vitha
PCR & spermidine kang at msvax.mssm.edu
OSTEOPONTIN and OSTEOCALCIN antibodies commercially available? G.E.Fazzi
PCR detection of viral RNA in whole blood specimens Paul Bumbak
MEDLINE. David J. Evans
PCR detection of viral RNA in whole blood specimens Paul Bumbak
PCR detection of viral RNA in whole blood specimens Paul Bumbak
Metabolic pathways poster? Tom Maynard
DNA prep for transient transfection nick at lmb1.rug.ac.be
In vitro transcription ...... r3211001
MS-DOS program for fragment sizing from digitisers Martin Trick
SDS Removal JFigue
Anti P-Ser/P-Thr Antibodies Stephan Klinz
direct sequence analysis of PCR products Kevin O'Connell
Human cDNA library in yeast expression vector. John P. Aris
Metabolic pathways poster? Andrew Berry
Request for Site directed mutagenesis Protocols (PCR) benedik at uh.edu
Electrophoresis Gel? David John Thornley
pET expression system Steve Minchin
size determination of rflps Ola
Univ. charging $100/mo./person for mainframe access Richard H. Miller
Bacteriophage Strain Collection Paul Hamilton
PCR of yeast gDNA. John Aris
Rhodamine-4-dUTP and biotin-11-dUTP Duncan Clark
G-C content determination paul betts
How to get rid of excess adaptors? John Dixon
alternative to silver stain? Christopher B. Yohn
Bal31 source Dr I.M. Clark
Human Cerebellum and / or Mouse Cerebellum c-DNA Mr Hassan Abdulrazzak
EtBr , How do you get rid of it? U12201 at uicvm.uic.edu
CALL: Peer Review Panel Recruitment: American Institute of Biological Sciences Keith L. Cowing
Transfecting Primary Cell Lines Emmanuel Skoufos, Ph.D.
problems with yeast two hybrid system ANDREI POPOV
Phosphorimager Questions Peter Heifetz
Slow PhosphoImager Bernard Khoo WCI
Electrophoresis Gel? tivol at tethys.ph.albany.edu
Univ. charging $100/mo./person for mainframe access Angela Werner
Kinase Substrate Sequences? PJOB
Staining nitrocellulose Ferenc Marincs
Wanted-Used Equipment Richard Schifreen
Can you "fix" agarose gels? Ferenc Marincs
How do I make a TA cloning vector? Markus Grompe, MD
Collegen assays a.vijayan
Univ. charging $100/mo./person for mainframe access mathog at seqaxp.bio.caltech.edu
TRANSFER OF PLASMIDS INTO BACTERIA GORDON BETTS
RefMan Rick Neubig
RefMan Rick Neubig
Kinase Substrate Sequences DREWES at MPASMB.DESY.DE
Phosphorimager Questions David Glover
small microfuges/quick spins? Daniel Kim
Pfu/Taq in PCR reactions Paul N Hengen
Restriction enzymes and methylation Daniel Kim
EtBr in partial digests of DNA Paul N Hengen
DNA elution from gels 4700gbera at umbsky.cc.umb.edu
Library rescue protocols Ian A. York
RNA electrophoresis ynakao at kean.ucs.mun.ca
Rhodamine-4-dUTP and biotin-11-dUTP Tony Murray
Bacteria cell lysis rogers elizabeth
Staining nitrocellulose *.*
Bacteriophage Strain Collection Reno Pontarollo
Metabolic pathways poster? dleviten at p001655.icos.com
Metabolic pathways poster? dleviten at p001655.icos.com
How do I make a TA cloning vector? Mike Hubank
Rhodococcus Phage Q4 Matthew Stoecker
methicillin&ampicillin kevin c. graham
Disposable filters for buffers etc Dan Diaz
DNA integration after transfection of mammalian cells Ian Lyons
DNA oligomers on acrylamide vs. agarose gel Nina Rosario L. Rojas
Anti P-Ser/P-Thr Antibodies AShaw5
Dyes and supercoiled DNA Brad Thomas
pET expression system bllim at hkucc.hku.hk
TA Cloning and screening Mike Poidinger
MEDLINE. Mike Poidinger
Re-using hybridisation solutions containing double-stranded probes? Void
Seeking ß-Gal plasmid with nuclear targeting signal! Sigrid Lehnert
RNA isolation from cryostat section KOH FURUTA
p53 gene theod at mel.dbe.csiro.au
p53 gene theod at mel.dbe.csiro.au
RNA isolation from cryostat section KOH FURUTA
p53 gene theod at mel.dbe.csiro.au
MAKING YOUR OWN GEL BOXES kang at msvax.mssm.edu
Highly competent cells kang at msvax.mssm.edu
Request for Site directed mutagenesis Protocols (PCR) kang at msvax.mssm.edu
Inosine in degenerate PCR primers? Hancock Lab
DNA integration after transfection of mammalian cells kang at msvax.mssm.edu
Transgenic mice; is it easy or difficult? kang at msvax.mssm.edu
smearing on pulse field gel GEN282E at ccs1.cc.monash.edu.au
Infection with nematode eggs Brendan Carson
A beginner in RACE PCR, or possibly inverse PCR needs advice! Richard Hastings
RNA from Liver Karl Fischer
ornithine decarboxylaseinhibitor louis at lmb1.rug.ac.be
SDS Removal Houthaeve Tony
test Helen Manley
methicillin&ampicillin Zal Suldan
HELP: streaks in isozyme electrophoresis Mikael Stenstrom
negative zymography Luigi Zampieri
(none) Gustavo Nolasco
MAKING YOUR OWN GEL BOXES Seth Corey
TRANSFECTION OF PLASMIDS INTO E. COLI GORDON BETTS
Pfu/Taq in PCR reactions Christopher Paul Palmer
P1 library Screening Marko Rehn
heteroplasmy of cloning vectors G
Phosphorimager Questions Mark W. Trumbore
Hyg assays in plants Jutta Tuerck Genetics
Church hyb solution questions Glen Shearer
Luminometer info? berickson at ntet.nctr.fda.gov
TRANSFER OF PLASMIDS INTO BACTERIA Martin D. Leach
In vitro transcription ...... Martin D. Leach
transfection of plasmids into E. Coli NEAL MUSTO
TRANSFECTION OF PLASMIDS INTO E. COLI Martin D. Leach
Univ. charging $100/mo./person for mainframe access Carlisle Landel
Looking for human aFGF and bFGF cDNA clones Dr. C.J. Chan
DNA Fingerprinting - Simpson Trial George Lin
ligation/transformation: HELP ME PLEASE!! HARDIES at THORIN.UTHSCSA.EDU
Disposable filters for buffers etc John A. Newitt
RE map software Karen Browning
Univ. charging $100/mo./person for mainframe access Candace Krepel
TRANSFER OF PLASMIDS INTO BACTERIA Kevin Oldenburg
used lab equipment Neal Stewart
TAQ ERROR RATES JIM
SKILLS REQUIRED BY CORPORATE LABS GORDON BETTS
TRANSFER OF PLASMIDS INTO BACTERIA David J. Westenberg
Mab for OspA of Borrelia???? Ali Karami
GST-Fusion zac
Molecular Biology Short Course?? joanna at WH.BAYER.COM
Mab for OspA of Borrelia???? Ali Karami
Is it Taq or the primer responsible for the mistake? carlos h. pedemonte
Changing RE to T/A Randall J. Cohrs
Phosphorimager Questions Malcolm Moos Jr.
alternative to silver stain? Malcolm Moos Jr.
How do I make a TA cloning vector? hodges bradley l
Anti P-Ser/P-Thr Antibodies Jim Woodgett
Pfu/Taq in PCR reactions hodges bradley l
Kinase Substrate Sequences Jim Woodgett
Rates of DNA oligo annealing Michael P. Frank
Univ. charging $100/mo./person for mainframe access tivol at tethys.ph.albany.edu
Phosphatase Columns Andrew G. McArthur
Is it Taq or the primer responsible for the mistake? Carlisle Landel
EDTA in hybridization? A. F. Cockburn
200 microliter m'fuge tube storage racks Jeff Rawlings
Microcentrifuge for Mol. Biol. -Best? Karl Fischer
DNA Strider Question Rick Conrad
TRANSFER OF PLASMIDS INTO BACTERIA Ian A. York
Disposable filters for buffers etc Mark W. Trumbore
Microcentrifuge for Mol. Biol. -Best? Brick Ayola
Question: What does BSA do in PCR reactions? Rick Conrad
EtOH precipitation Rick Conrad
pfu polymerase Michael Gale
Pulse field gels biukovic at olimp.irb.hr
Is it Taq or the primer responsible for the mistake? benedik at uh.edu
EtOH precipitation benedik at uh.edu
SDS Removal/hemolytic protein Karl Fischer
Denaturing ds DNA for sequencing benedik at uh.edu
One step transformation "Mayer, Jorge ", 3637
Univ. charging $100/mo./person for mainframe access Lisa
Issue 1 of new U.K oriented DIAGNOSIS magazine Roger Moreton
Okazaki fragments -- in Thermicus aquaticus? Rob Britton
DNA Fingerprinting - Simpson Trial U27111 at uicvm.uic.edu
One step transformation kang at msvax.mssm.edu
PCR & spermidine Shiao Y. Wang
heteroplasmy of cloning vectors "Alexander Kraev" bckraev at aeolus.ethz.ch
Accession numbers for human JAKs and STATs FNINER
Squalene/H2O2 reaction - study.txt [1/1] Ted Kalli
methicillin&ampicillinREGISTER kitchingman at mbcf.stjude.org
E.coli "gene knockout": help Giuseppe Borsani
alternative to silver stain? U12201 at uicvm.uic.edu
size determination of rflps U12201 at uicvm.uic.edu
hIgG1 plasmid availability Gwen Shafer
Microcentrifuge for Mol. Biol. -Best? Brick Ayola
EtBr in agarose gels vs. EtBr staining later Karl Voss
hIgG1 plasmid ANDREI POPOV
Hybridization oven Theo Devos
GST-Fusion R. Rex Denton
ATCC Catalogs? Torsten Borchers
Is it Taq or the primer responsible for the mistake? Robert Woodward
EtBr in agarose gels... Ian Lyons
SKILLS REQUIRED BY CORPORATE LABS XYZZYX at DELPHI.COM
EtBr staining: A third option BCRUBIN at weizmann.weizmann.ac.il
Direct sequencing of PCR products BCRUBIN at weizmann.weizmann.ac.il
(none) Sei-Heon Jang
Denaturing ds DNA for sequencing Mr SMNN Faruque
PCR & spermidine Dr Keith Anthony Grimaldi
Genomic DNA from cells (re: Kits) Dr Keith Anthony Grimaldi
Introducing a single pyrimidine dimer into a DNA sequence Andrew Akerman
EtBr in agarose gels... Robert Preston
ATCC Catalogs? Francis Ouellette
(none) Gustavo Nolasco
primer for RT-PCR Steven Sullivan
RT-PCR of Rare mRNA Steven Sullivan
Question: PCR With 500bp Primer? Steven Sullivan
Question: What does BSA do in PCR reactions? Steven Sullivan
Long PCR hints -- any sources? Steven Sullivan
ligation/transformation: HELP ME PLEASE!! Klaus Salger
DNA Fingerprinting - Simpson Trial Richard E. Showalter
DNA Fingerprinting - Simpson Trial Richard E. Showalter
Phosphorylated amino acids? Dynagen at rt66.com
digital gel documentation Johnny Wharton
Membrane oxidoreductase on sperm? Jim Cummins
digital gel documentation Peter Heifetz
Total cellular protein vs cell number Stephen Gately
Ligation of alkaline phosphatase treated DNA kang at msvax.mssm.edu
control for TPA induction L.Y.Chau
MICROSATELLITE ISOLATION Philip OBrien
Total cellular protein vs cell number Yasuhito Abe
Attention Agnes Tay Hou Ngee contente at usuhsb.usuhs.mil
primer for RT-PCR Shahram Mori
EtBr in agarose gels... Shahram Mori
Question: PCR With 500bp Primer? Shahram Mori
Dyes and supercoiled DNA Andy Bates
Radiolabelled Novobiocin sbaker at molbiol.ox.ac.uk
RFLP subtraction Takashi Hashimoto
Pfu/Taq in PCR reactions Duncan Clark
How to get rid of excess adaptors? HedstromR
Church hyb solution questions Anthony Tomlinson
Drosophila actin probe for RNase protection wanted Bernard Khoo WCI
Plasmid Diagrams Neil Harris
Enhancement of PCR? Richard Hastings
GFP-Vectors with oriP ? thoenes
E.coli "gene knockout": help Robert Solomon Bioc
Help for ApG !! yhping at ccvax
Blot pictures vitor warwar
Okazaki fragments -- in Thermicus aquaticus? Zophonias O. Jonsson
Accession numbers for human JAKs and STATs Seth Corey
IGFBP-3 ynakao at kean.ucs.mun.ca
Ligation of alkaline phosphatase treated DNA Andy Bates
Church hyb solution questions Warren Gallin
primers in RT in situ PCR Rob Wesley
Denaturing ds DNA for sequencing "Alexander Kraev" bckraev at aeolus.ethz.ch
Okazaki fragments -- in Thermicus aquaticus? Duncan Clark
Who sells "UNGRAPH" software? bwr at gnv.ifas.ufl.edu
Ligation of alkaline phosphatase treated DNA David Micklem
Re-using hybridisation solutions containing double-stranded probes? Jim Litts
Electrophoresis Gel? Chris Boyd
DNA prep for transient transfection seppen at amc.uva.nl
human neurofilament (NF-H) Randall J. Cohrs
DNA prep for transient transfection Ian A. York
Companies with E-mail/Gopher/WWW U12201 at uicvm.uic.edu
EtBr in agarose gels vs. EtBr staining later David L. Haviland, Ph.D.
UV Crosslinking Protein/DNA Linda M Ritter
A beginner in RACE PCR, or possibly inverse PCR needs advice! paiks at gunet.georgetown.edu
Acetyl Coenzyme A Michael Berryhill
Plasmid Diagrams Pearse Ward
Need antibodies for ompA protein taylor
Peptides on the head of a Pin? JoannePetithoryjpetithory at genencor.com
SYBR green Mauro
Stable episomal vector in mouce cells bartd at lmb1.rug.ac.be
DNA prep for transient transfection
Mobility Shift and Glutamic Acid Stefan Einar Stefansson
Total cellular protein vs cell number Jeff Rawlings
PCR using lambda gt11 primers elida at parasit.lan.mcgill.ca
Nucleic acid modifications Rick Conrad
progesterone responsive rat RNA probe Mike Gruidl
Plasmid Diagrams Karen Usdin
Definition: Saturation Mutagenesis Eric C. Anderson
TAQ genomic library?? Wendy Bedale
who sells cheap MOPS. PLEASE.****** Shahram Mori
Drosophila actin probe for RNase protection wanted Shahram Mori
Enhancement of PCR? Shahram Mori
pfu polymerase Yi, Xiaoming
primers in RT in situ PCR Shahram Mori
Q:Does Gene Clean interfere with ligation? Yi, Xiaoming
random hexamers as primers for RT in RT-PCR Robert Hewitt
GST-Fusion I-Jen Huang
Phosphorimager Questions neale at mbcf.stjude.org
Hybridization Protection Assay Udo Reischl
MULV-RT Ist. Cibernetica - Rep. Sistemi Cellulari
Taq extenders and Pfu polymerase Your Name Here
Precut nylon hybridization membranes? Jarkko Kortesmaa
Plasmid Maps R. John Lye
Replica plating of cells Peter J. Hornsby
Rf: Precut nylon hybridization membranes? LOGAND
ligation/transformation: HELP ME PLEASE!! Shin Enomoto
3D tumor culturing Pieter Proot
Companies with E-mail/Gopher/WWW Mike Agostino
COS-1 cells wanted seppen at amc.uva.nl
Total cellular protein vs cell number Warren Gallin
DNA prep for transient transfection Ken Saville
Total cellular protein vs cell number ggza at umich.edu
MULV-RT Parke K. Flick
Taq extenders and Pfu polymerase Parke K. Flick
Disposable filters for buffers etc Tracy Aquilla
Okazaki fragments -- in Thermicus aquaticus? Duncan Clark
Tm of a DNA hairpin HARDIES at THORIN.UTHSCSA.EDU
oligonucleotides DJC poland
DNA prep for transient transfection mrb at WH.BAYER.COM
PCR with 500bp Primer HARDIES at THORIN.UTHSCSA.EDU
Companies with E-mail/Gopher/WWW Martin D. Leach
DNA prep for transient transfection Martin D. Leach
who sells cheap MOPS. PLEASE.****** Martin D. Leach
Precut nylon hybridization membranes? Martin D. Leach
Precut nylon hybridization membranes? Martin D. Leach
who sells cheap MOPS. PLEASE.****** Sean David Moore
Enhancement of PCR? (by boiling primers) HARDIES at THORIN.UTHSCSA.EDU
random hexamers as primers for RT in RT-PCR Sean David Moore
MULV-RT Sean David Moore
size determination of rflps Dr. J.P. Clewley
TRANSFER OF PLASMIDS INTO BACTERIA BIO101 at DELPHI.COM
On/Off rates of Rec. Fabs? Mxm
oocyte expression cloning Adam S. Asch
digital gel documentation Christopher B. Yohn
Definition: Saturation Mutagenesis Rock Pulak
unsubscribe picatags at TCPLINK.NREL.GOV
control for TPA induction Manuel Maria SIMON
Precut nylon hybridization membranes? Silvia Spitzer
M-MLV Neil Harris
Q:Does Gene Clean interfere with ligation? Richard R. Hardy
Oligo Question Andy Kravetz
DNA Strider GORDON BETTS
Kits for genomic DNA from blood (lymphocytes) Brad Thomas
Mobility Shift and Glutamic Acid Ken Doyle
transfection of keratinocytes Alexis Harington
who sells cheap MOPS. PLEASE.****** Stephen P. Driska PhD
detecting pcr-products by hplc Holger Bohnemeier
Ligation of alkaline phosphatase treated DNA Martin Kennedy
Phosphorimager Questions Tracy Aquilla
RE map software Aurel Yang
anti-mouse integrin/neutrophil mAb Douglas Ormrod
Oligo Question Rob Britton
Precut nylon hybridization membranes? Dr. Kent L. Nastiuk
DNA Strider Marc Goldstein
T7 RACE? Mark Fry
ligation/transformation (type I restriction) HARDIES at THORIN.UTHSCSA.EDU
REVISED SOFTWARE: Random Cladistics 2.1.1 Mark Siddall
Total cellular protein vs cell number AShaw5
Q:Does Gene Clean interfere with ligation? Simon Dawson
Two-hybrid query Simon Dawson
Rubisco clone Alan Schulman
Church hyb solution questions "Alexander Kraev" bckraev at aeolus.ethz.ch
Sequencing apparatus bjvv at scinet2.up.ac.za
Plaque PCR mystery "Alexander Kraev" bckraev at aeolus.ethz.ch
Anion/Cation free agar Neil Harris
ligation/transformation: HELP ME PLEASE!! Alan Lyons
extraction of DNA from blood clots MIRIAM P. ROSIN
Plasmid Diagrams Tony Hodge
Church hyb solution questions David Micklem
Re-using hybridisation solutions containing double-stranded probes? Jan Eggermont
Transfecting Primary Cell Lines Jan Eggermont
RAPD with Air Thermal Cycler UDAY PATHRE
Restriction Digestion and Bacteria GORDON BETTS
Electrophoresis Gel? Ken Saville
Sequencing apparatus Martin D. Leach
(none) Jeff Kramer
mouse vs human Jeff Kramer
OXYGENE DALLAS? Doug-young Ryu
Promoter recognition and Alu masking Giuseppe Borsani
Agarose Gel Loading Dye hAPPY gUY
Wanted Verticillium agaricinum Anthony Hall
Church hyb solution questions Edwin Fink
How many DNA binds to Nylon membranes Dietmar Baas
Promoter recognition and Alu masking Giuseppe Borsani
looking for a rat genomic library Giuseppe Borsani
Epitope tagged molecules David L. Haviland, Ph.D.
Two-hybrid query
Buying a thermocycler Gregory E. Gonye PhD
Long PCR with Taq and Vent quin chou
AZTREONAM, HELP! chengs1 at jaguar.uofs.edu
Yeast plasmids sought Peter J. Russell
Antibody for detecting murine mdm-2 Annette C. Hollmann
oocyte expression cloning Michael Coady
WHO SELLS EXO-KLENOW??? Darren M. Stauth
Research mice & where should they come from? GORDON BETTS
Kits for genomic DNA from blood (lymphocytes) Silvia Spitzer
CO2 Cell Culture Incubator Anton Tevelev
digital gel documentation Michael A. Shia
2-D Electrophoresis ya-ya
pGEX (GST) expression system bipin dalmia
EtBr in agarose gels vs. EtBr staining later Rae Nishi
CAT assays in E.coli Albert Crescenzo
RT-PCR of Rare mRNA Rae Nishi
alternative to silver stain? Torsten Borchers
Drosophila actin probe for RNase protection wanted Richard M. Rohan
pedigree software JOHN A. KORTE
EtOH precipitation Jim Litts
Enhancement of PCR? Michael Cooley
CAT assays in E. coli Albert Crescenzo
la pcr complexes aEscher
Re-using hybridisation solutions containing double-stranded probes? Glen Shearer
DMSO in PCR rxns ?? Glen Shearer
subscribe Christopher Thomas Richie
Stable episomal vector in mouce cells mpk6
GFP in CHO cells ICAgen
WHO SELLS EXO-KLENOW??? Sunil Shaw
On/Off rates of Rec. Fabs? H. David Fischer
Research mice & where should they come from? John Ladasky
Introducing a single pyrimidine dimer into a DNA sequence Scott Keeney
Qwik-precip Ann Schofield
Preparartion of Liver DNA for PFGE Dr Saleem Mohammed
BbvII Restriction enzyme Klaus.Matthaei at ANU.EDU.AU
WHO SELLS EXO-KLENOW??? Dan Diaz
Monteray GFP meeting Kendall
ligation/transformation: HELP ME PLEASE!! Steven Loechel
EtBr , How do you get rid of it? Greg Shen
Fluoro-dATP sequencing kit problem Jarkko Kortesmaa
plasmid isolation from yeast Marco Gagiano
ligation/transformation: HELP ME PLEASE!! "Alexander Kraev" bckraev at aeolus.ethz.ch
DMSO in PCR rxns ?? Matthias Zeiner
DMSO in PCR rxns ?? Glibert Fabrice
why fix sequencing gel? Anne Bazanowski
Sequencing apparatus R. John Lye
Church hyb solution questions "Alexander Kraev" bckraev at aeolus.ethz.ch
How "random" are random primers? mdcabl at cc.newcastle.edu.au
Conservation of extracellulaire matrix mazella at naxos.unice.fr
Need connexin 26 antibody Dooyeon Kim
Enhancement of PCR? Zal Suldan
RNA isolation from cryostat section Koh Furuta
WHO SELLS EXO-KLENOW??? Parke K. Flick
eosophageal cells Alan Thornley
GFP review PRASHERD at DELPHI.COM
la pcr complexes (a network) HARDIES at THORIN.UTHSCSA.EDU
phosphorothioate oligos for antisense Dave Keys
How "random" are random primers? Richard T. Pon
little blue homogenisers Peta C. Bonham-Smith
BbvII Restriction enzyme Rick Smith
WHO SELLS EXO-KLENOW??? Rick Smith
Rf: Re: Enhancement of PCR? LOGAND
Rf: How "random" are random primers? LOGAND
cloning with PCR Sheris at OSUUNX.UCC.OKSTATE.EDU
Random hexamers (again) LOGAND
little blue pestles Jillian Johnston
little blue homogenisers David R. Rockhold
What's the most efficient way to remove DNA from polacyrlamide gels? John Wurzelmann
Temperature gradient gel electrophoresis (TGGE) r jones
little blue homogenisers GANESHAN at SASK.USASK.CA
potato tuber RNA? Gellatly Kevin S
SIB acronym dtk
Nla IV - Restriction Enzyme stephen marshalko
Church for Northern blots? Manuel Maria SIMON
mouse vs human Emmanuel Skoufos, Ph.D.
Promoter recognition and Alu masking Emmanuel Skoufos, Ph.D.
detecting pcr-products by hplc Peter Doris
pWE15 screen Tanya Reichert
test Greg Warren
test Greg Warren
why fix sequencing gel? kang at msvax.mssm.edu
gene expression in neurons Fiberman
GPI anchors H. David Fischer
PCR using lambda gt11 primers jfh
Where to get vector phGHam-g3? Daniel Kim
plasmid isolation from yeast Dirk Truman
transient transfection Seth Corey
RT-PCR Monica J. Martick
pcDV1 - what is it? Ian A. York
Trizol for total RNA M.Johan Broekman
Wooden Toothpicks (Taq Inhibitor) Paul N Hengen
Pfu/Taq in PCR reactions pgegen at rnaworld.bio.ukans.edu
Oligo 4.0-s program SPAGNOL at PLAUTO.CSATA.IT
Software for interpruting restricton digests. Larry Reiter
Qiagen preps in transient transfections are OK Ian A. York
Epitope tagged molecules Richard T. Timmer
Oligo 4.0-s program Klaus.Matthaei at ANU.EDU.AU
little blue pestles Paul D. Boyer
X-ray film storing Rafael Maldonado
Gel box less than 1 dollar kang at msvax.mssm.edu
Antibiotic shelf life? William C. (Bill) Johnson
Graphing Programme Shiao Y. Wang
AMOUNT OF PRIMER IN DNA FINGERPRINTING Shiao Y. Wang
Autoclaving Dextran Sulfate? Shiao Y. Wang
QIAquick Spin Columns Pekka Rappu
PCR with few target on spinal fluid Dick Nelson
Killer gene Pekka Rappu
P1 library Screening Daniel L. Burgess
Nla IV - Restriction Enzyme Tim Hutchin
mouse vs human Daniel L. Burgess
DNA comparison Gbolagade Babalola
Inverse PCR on cDNA Gordon Stott
eosophageal cells John Fox
CO2 Cell Culture Incubator Manuel Maria SIMON
Phenol distillation Roy Dace
Toothpicks for PCR "Alexander Kraev" bckraev at aeolus.ethz.ch
antibody against fibronectin receptor Shao-Yu Chen
why fix sequencing gel? "Alexander Kraev" bckraev at aeolus.ethz.ch
NIH Phone Number Doug-young Ryu
Church for Northern blots? Randy Haun
Oligo 4.0-s program Simon Dawson
DNA FROM WHOLE BLOOD I.Ridgers at nhm.ac.uk
Chelex protocol? Karen B. O'Brien
UV crosslinking ynakao at kean.ucs.mun.ca
Does Klenow proofreading multiple-base 3' overhangs or just single base? Pete Muriana
Quantitative PCR Rajesh R. Naik
Phenol distillation Shaun D. Black
in situ, ethanol or methanol for dehydration? jie li
transient transfection Emmanuel Skoufos, Ph.D.
Sequencing Gels Reid, Thomas M.
E.coli "gene knockout": help Richard_Heath
X-ray film storing Emmanuel Skoufos, Ph.D.
MeOH in Western Buffer Louis Hom
FTP site for FAQ list and TIBS manuscripts restored Paul N Hengen
*** Unscheduled DNA synthesis? *** KGEISS%RAVEN at EAGLE.AAMRL.WPAFB.AF.MIL
*** Unscheduled DNA Synthesis? *** KTGEISS at MIAVX1.ACS.MUOHIO.EDU
Nla IV - Restriction Enzyme Keith G. Weinstock
Help needed-- understanding POROS / Perfusion chromatography RMARTINQFD at BIOTECHNET.COM
NIH Phone Number John A. Newitt
NIH Phone Number Zal Suldan
ELISA for human IgG ANDREI POPOV
Church for Northern blots? Glen Shearer
Recombinant Circle PCR Daniel Kim
eosophageal cells John Fox
Phenol distillation Jim Owens
Toothpicks for PCR Darren Boehning
UV damage, dephosphoryl., and making constructs: MAYBE it's SOLVED??? Heidi Moss
Want PCR to incorporate 32P label PAUL JONES
pre-cut culture dish membranes Deborah Lynn William
Any pACYC plamids with T7 promoters? Scott Taylor
Rapid Polyacrylamide Polymerization Victor Defilippis
test, disregard Robert Farese
EtBr decontamination NATHALIE RANCIAT
n Karen B. O'Brien
Fluoro-dATP sequencing kit problem Casey Chin
Does Klenow proofreading multiple-base 3' overhangs or just single base? Pamela Norton
Want PCR to incorporate 32P label kang at msvax.mssm.edu
Methylmercuric hydroxide- SOURCE? rosemary hoffman
Does Klenow proofreading multiple-base 3' overhangs or just single base? brett at BORCIM.WUSTL.EDU
DMSO in PCR rxns ?? jie li
CO2 Cell Culture Incubator Virginia Dress
CO2 Cell Culture Incubator Brad Thomas
Rapid Polyacrylamide Polymerization Brad Thomas
anti-centromere antibody? source? J.Carnwath
CO2 Cell Culture Incubator Cheah Kathryn Song Eng
4-aminobutyric acid assay Mohohlo Molatudi Botany
CMC of Octyl-POE? Moritz v.Rechenberg
One-step inactivation of RNase in Ambion RPA kit Bernard Khoo WCI
Ligation problems Francois Nantel
High mol wt bFGF bands-repost N.Ambalavanan MD
An IPCR Tale SPAGNOL at PLAUTO.CSATA.IT
PCR trouble with Arabidopsis gene Bert Popping
Biotech Company WWW registry Martin D. Leach
Ligation problems brett at BORCIM.WUSTL.EDU
UK oriented lab digest (Diagnosis) on WWW - ng-info.txt [1/1] Pam Moreton
Antibiotic shelf life? Curt Ashendel
Plaque PCR mystery Wen-Yen Kao
Antibiotic shelf life? John Brunstein
UK oriented lab digest (Diagnosis) on WWW - ng-info.txt [1/1] Roger Moreton
E.coli "gene knockout": help Chris Yost
Antibiotic shelf life? Emmanuel Skoufos, Ph.D.
Ligation problems Emmanuel Skoufos, Ph.D.
lambda insert isolation Cheung Cho Yue, M.D.
who sells cheap MOPS. PLEASE.****** Shahram Mori
random hexamers as primers for RT in RT-PCR Shahram Mori
Ru 486 Season Rose
GST-Fusion Shahram Mori
la pcr complexes Shahram Mori
Rf: How "random" are random primers? Shahram Mori
Phenol distillation JAG at QUCDN.QueensU.CA
Phenol distillation Shahram Mori
Want PCR to incorporate 32P label Shahram Mori
E.coli "gene knockout": help Rafael Maldonado
Proteinase K SPAGNOL at PLAUTO.CSATA.IT
Amplicon's purification. SPAGNOL at PLAUTO.CSATA.IT
GPI anchors Iain Wilson
Source of SceI LONSDALE
HELP ME!! (lig/trans: UV and CIP) -- repeat message Heidi Moss
Restriction Digestion and Bacteria Richard_Heath
E.coli "gene knockout": help Brad Nicholson
Need Input on Northern With Bacterial RNA Christine Ward
Killer gene Bernard Heymann
Source of SceI ANDREI POPOV
biotin-avadin in IP Xiangjun Zhou
P1 Screening/isolation Amos Heckendorf
Help needed-- understanding POROS / Perfusion chromatography Amos Heckendorf
MeOH in Western Buffer Malcolm Moos Jr.
Oligo 4.0-s program Pierre_Talbot at Infopuq.UQUEBEC.CA
little blue homogenisers
why fix sequencing gel? Daniela Sciaky
TEST anonymous at somehost.somedomain
HISTONS / who is (Your Name)? Daisuke Umeno
Fructans and fructose? A.Marchant
Ab for ferritin? L.Y.Chau
can formamide harbour RNases? Shahram Mori
Q : E. coli instability Elizabeth Lawrence
genomic dna Mr Vid Mohan-Ram
Need Input on Northern With Bacterial RNA Shahram Mori
SV40 HANELT
Sequencing Gels "Alexander Kraev" bckraev at aeolus.ethz.ch
UV damage, dephosphoryl., and making constructs: MAYBE it's SOLVED??? "Alexander Kraev" bckraev at aeolus.ethz.ch
Killer gene mpage at molbiol.ox.ac.uk
DNase treatment of RNA sample Toshiharu Ishizuka
DMSO in PCR rxns ?? Matthias Zeiner
HELP ME!! (lig/trans: UV and CIP) -- repeat message -Reply David Osterbur
MeOH in Western Buffer Matthias Zeiner
phosphorothioate oligos for antisense Jim Burrows
Any pACYC plamids with T7 promoters? Randy Haun
PCR using lambda gt11 primers Malcolm Moos Jr.
Gel box less than 1 dollar Sean David Moore
Gel box less than 1 dollar Nsubuan Johnson
Want PCR to incorporate 32P label Manuel Maria SIMON
Linear Polyacrylamide (Ian Ridgers)
GST-Fusion ossipow
SYBR green Sandy Silverman
pLysS freezer stock instability Frank van de Loo
help me!! (254 nm UV damages DNA) HARDIES at THORIN.UTHSCSA.EDU
Toothpicks for PCR Guan Zhu
Oligo 4.0-s program (how to adjust parameters) HARDIES at THORIN.UTHSCSA.EDU
DHFR and methotrexate jeg at PW.USDA.GOV
Pure bacterial chromosomal DNA Alejandro Abuin
mouse vs human Tracy Aquilla
Non-radioactive cDNA library screening Bernd Stein
Elution from Glutath. beads ossipow
Genetic Diagnostics/Infectious Diseases Conference Cambridge Healthtech Institute
DNA Preparations John Fox
ASCII file of molecular biology words Paul N Hengen
HELP ME!! (lig/trans: UV and CIP) -- repeat message Joel A. Kreps
EtBr , How do you get rid of it? J.Carnwath
The One-dollar Gel Box Daniel Kim
CAT assays in E. coli rogers elizabeth
RNAse protection assay Katherine Schaefer
Charon 4a Yamamoto
SYBR Green Jfloring at aol.com
Proteinase K Klaus Salger
COS-1 cells wanted Uttam Rajbhandary
Old World Monkey cell line sources? Paul Edward Kowalski
FOR SALE: Discount Lab Glassware John Brookes
Methylmercuric hydroxide- SOURCE? Thomas O'Neill
sequence programs Daniela Sciaky
TA cloning -Promega Edward Wang
software to generate restrcition fragment graph Aurel Yang
DHFR and methotrexate Thomas O'Neill
CD4 coding sequence- commercially available? ICAgen
Large Scale Insect Cell Culture BIOTEC54
Want PCR to incorporate 32P label Dr. Kent L. Nastiuk
Bal31 source BIOTEC54
M-13 kits Claude Baker
M-13 Kit Claude Baker
Request for Site directed mutagenesis Protocols (PCR) Hooperkp
Cracked SSCP gels r.j. dewar
suggestions on automated DNA sequencer? Hong Yan Dr
Killer gene again Pekka Rappu
mouse vs human bcdlee at hkpucc.polyu.edu.hk
suppliers of Fse1,Sse83871, BspG1 ??? HARVEY
CO2 Cell Culture Incubator (Dave Johnston)
How many DNA binds to Nylon membranes Anthony Tomlinson
Q : E. coli instability "Alexander Kraev" bckraev at aeolus.ethz.ch
Ligation problems Anthony Tomlinson
DNase treatment of RNA sample Toshiharu Ishizuka
Want PCR to incorporate 32P label Zal Suldan
Elution from Glutath. beads Zal Suldan
STR systems Wasun Chantratita
in situ labs? Christiane=Frahm at rz.hu-berlin.de
Does Klenow proofreading multiple-base 3' overhangs or just single base? Tracy Aquilla
anti-murine TGFbeta2 antibody Torreele Els
CD4 coding sequence- commercially available? Luis M. Alvarez
MeOH in Western Buffer John Fox
Proteinase K Mail at scri.sari.ac.uk
Phenol distillation 4700gbera at umbsky.cc.umb.edu
Cellulose capsule removal from Acetobacter Labuser 1
Retrieval of ssDNA from a mixture of ssDNA/dsDNA Mr. J. Sasse
Methylmercuric hydroxide- SOURCE? Thomas O'Neill
RNAse protection assay Emmanuel Skoufos, Ph.D.
FOR SALE: Discount Lab Glassware Sean David Moore
Recombinant Circle PCR Don Jackson
Microwave techniques? Scott K. Boyer
TA cloning -Promega Paul N Hengen
RNA secondary structure programs your_mcmail_id
EtBr decontamination Paul N Hengen
Phenol distillation Edgar Schreiber
RNAse protection assay Pamela Norton
Cell line expressing sLex antigen BMA14432 at vax1.utulsa.edu
Church hyb solution questions Glen Shearer
pLysS freezer stock instability Richard_Heath
Killer gene Rafael Maldonado
Pure bacterial chromosomal DNA Rafael Maldonado
Two hybrid Q. Rafael Maldonado
mouse vs human jfh
COS-1 cells wanted Bernard Murray
DNase treatment of RNA sample Virginia Dress
lyophilization of antisera nandid at ucbeh.san.uc.edu
IN SITU RT-PCR Agnieszka Szczepek
Two hybrid Q. Bernard Murray
Chromosome seperation Frank Narz
GST-Fusion L Bruce Sk
Amino Acid composition? lmjakt at hkucc.hku.hk
Amino Acid composition? pathology at mscf.med.upenn.edu
WANTED: Course lab protocols Garry P. Larson
Drosophila FM6 balancer problem Miss Hong Saw Tin Amy
MULTIPLE SCLEROSIS CONFERENCE irc at III.NET
taq preparation Prasit Palittapongarnpim
0.2-mL PCR tubes John Braverman
HepG2 transfection w/liposomes Vennie
pWE15 Cosmid vector D L Clarke
pWE15 Cosmid vector D L Clarke
Autoclaving Dextran Sulfate? Dr Joseph Behnam
neuronal cell line (guinea pig) Thomas Loeffler
COS-1 cells wanted Randy Haun
suppliers of Fse1,Sse83871, BspG1 ??? Duncan Clark
Favorite bacterial RNA prep?? Melanie Jean Barnett
DNA FROM WHOLE BLOOD (Ian Ridgers)
Retrieval of ssDNA from a mixture of ssDNA/dsDNA Allen Gathman
ELECTROPORATION PARAMETERS FOR LETTUCE PROTOPLASTS NICOL,NATHALIE,MS
suppliers of Fse1,Sse83871, BspG1 ??? Roger F. Anderson
Getting out the brown from DNA Richard Dana
apoptosis detection: problems with test kits Inst. for Biochemistry
pWE15 Cosmid vector Francis Ouellette
MeOH in Western Buffer Daniel Kim
Large Scale Insect Cell Culture bipin dalmia
Drosophila FM6 balancer problem Ken Saville
Ligation problems "Alexander Kraev" bckraev at aeolus.ethz.ch
Two hybrid Q. Erica Golemis
Pischia expression Jonathan Kurtis
Taq-Pfu compatibility SPAGNOL at PLAUTO.CSATA.IT
Regulated expression cloning vectors HELP Edgar Valencia
CAT assays in E. coli Michael Cooley
Toothpicks for PCR Michael Cooley
SV40 Daniel, Phil
CAT assay problem! jr at dna.bio.warwick.ac.uk
Stratagene's Quik Hybridization solution books at mcvax2.d48.lilly.com
want an ABI 380B DNA synthesizer Jeffrey Chang
READ/NEW/FOLLOWUP gough_k at wehi.edu.au
IMPORTANT!! - MAIL QUEUE PROBLEMS AT NET.BIO.NET BIOSCI Administrator
JBC journal (3.3.95) marcvdc at lmb1.rug.ac.be
culturing spermatozoa Harry J. Banaharis
Microwave techniques? Scott Belcher
Stratagene's Quik Hybridization solution Jun IKEDA
PCR enz:Taq/Pwo Doug-young Ryu
Request for actin clone (?) pzhang
M-13 Purification kits Claude Baker
HYbridization Incubator bcdlee at hkpucc.polyu.edu.hk
SV40 Giovanni Maga
Advice on mitochondria isolation from fungi (i.e. percoll gradient). Gus Trautweiler
Source of SceI Javier PEREA
HELP: Microtubuli or tubulin Thomas Mohr
HYbridization Incubator Simon Baker
Ligation problems Stephen Gately
D-aspartate in bacteria Marc DIEU
Specificity of endoproteinase Glu-C Marc DIEU
Molar extinction coefficient of a protein at 280 nm. Marc DIEU
Electrophoresis Gel? RandBio
Request for actin clone (?) Martin D. Leach
Autoclaving Dextran Sulfate? Stephen R. Lasky, Ph.D.
Oligo 4.0-s program Tracy Aquilla
DNase treatment of RNA sample Tracy Aquilla
DNase treatment of RNA sample Jim Owens
In Situ Hybridization Brian Halloran
Getting out the brown from DNA 4700gbera at umbsky.cc.umb.edu
Pischia expression RCT2BNC
Autoclaving Dextran Sulfate? Alan Gerstein
Oligo 4.0-s program Lisa Bibbs
protein kinase A inhibitors Stephen Amato
BAC library construction, I need help! SYME,JENNIFER ALBERTA,MS
VectorNTI frits at hubrecht.nl
M-13 Purification kits "Alexander Kraev" bckraev at aeolus.ethz.ch
ligation problems HARDIES at THORIN.UTHSCSA.EDU
microwave digests (hmmm) John Dixon
Electroporation of RNA in cos-cells alechi at BIO.EMBNET.SE
RNA isolation kits (G+) jtbrown at osuunx.ucc.okstate.edu
CAT assays in E. coli rogers elizabeth
Stratagene SurfZAP phage display vector Daniel Kim
ECO-STAR John and Alex
Strange PCR colony screen result Eric C. Anderson
G0 antibody Koh Furuta
why fix sequencing gel? Kirk Bartholomew
PCRs no longer working. HELP! John Compton
Q: Direct PCR prod. sequencing Eric C. Anderson
Elution from Glutath. beads Dennis Templeton
Microwave techniques? John Hodgson
The One-dollar Gel Box-Graphite plates for blotting Dennis Templeton
Anyone using Ericomp thermal cyclers? John Hodgson
Transfection efficiency Dave Bates
VectorNTI R. John Lye
Toothpicks for PCR Robert Preston
apoptosis detection: problems with test kits SHO1
Transfection efficiency Ian A. York
M-13 Purification kits Luis M. Alvarez
antibody compatibility Fiberman
Xenopus liver cDNA library elder at CGL.UCSF.EDU
Will I lose half my adaptor by gel purifying? John Dixon
Multiple IgG bands in reduced SDS-PAGE Dayanidhi Raman
PCR primer design Pierre_Talbot at Infopuq.UQUEBEC.CA
Will this experiment work? (p53-related) aEscher at ccmail.llu.edu
Cloning using lamda-phage TA cloning kit Andrew Parolin
Ligation problems wmbonner at helix.nih.gov
MJ PCR machines and BioRad gel doc systems Cheah Kathryn Song Eng
Who sells microsatellite primer/ probe kit? huangbx at DEAKIN.EDU.AU
Competent Yeast Cells Dirk Truman
Stratagene SurfZAP phage display vector Shahram Mori
SYBR and gel staining Ian Clements
Pischia expression PWHoward
Returned mail: research in flavors anantha r kolleivgode
Molar extinction coefficient of a protein at 280 nm. Ralf Morgenstern
"missing" RNA Karen Ferri
SV40 (fwd) Giovanni Maga
Freezing Bacteria-Liquid N2 OK? Kimberly Baldwin
2-D Electrophoresis P. Friedl
Km of glutamate dehydrogenase enrcaslt at eucmvx.sim.ucm.es
Why can't I get agarose embedded HMW DNA to digest ? cavell at BBSRC.AC.UK
Analysis of 2D-gels with scanner and PC P. Friedl
immunofluorescence background Dave Gilbert
Complement and plastic microtiters Luca Jovine
Competent Yeast Cells Zal Suldan
Molar extinction coefficient of a protein Alan Cooper
Misincorporation rates of polymerases Uwe Harmening
Fructans and fructose? bjorn
BSA Tom Young
Measurement of cholesterol in mice Lopez-Molina Luis
Pischia expression RCT2BNC
Cholesterol-rich food for mice Lopez-Molina Luis
DNase treatment of RNA Jillian Johnston
why fix sequencing gel? K. Legate
CAT assay problem! Manuel Maria SIMON
RNAse protection assay FIXED! Katherine Schaefer
PCR primer design Jim Owens
Southern with small probe? John Dixon
Why can't I get agarose embedded HMW DNA to digest ? Howard T. Petrie
Autoclave to remove RNAses. TUCKER at AFP76.HUMC.EDU
Methylmercuric hydroxide- SOURCE? Nancy Lemieux
Microwave techniques? Eric C. Anderson
Freezing Bacteria-Liquid N2 OK? Aaron Pawlyk
Q about boiling miniprep sequencing. kang at msvax.mssm.edu
Inducible mammalian expression vectors mammalian Alan Thornley
Toothpicks for PCR "Alexander Kraev" bckraev at aeolus.ethz.ch
Ligation problems John and Alex
immunofluorescence background Richard R. Hardy
Sticky DNA after Hind III-Why? LOGAND
CO2 Cell Culture Incubator John Wolff
Phenol distillation John Wolff
(none) HOWARD at MGRP.BGSM.WFU.EDU
Carboxy-terminal Antibody to gp120 Richard Mandel
Leukemia Res. Conf. in Chicago (9/95) Eric Bremer
A.S.A.P GENOMIC DNA ISOLATION KIT Ketil Thorstensen
alignment software DJC poland
RT-PCR using S1 Nuclease Petra M. Jakobs, Ph.D.
NEEDED: micro-solid phase extraction column Michael A. Strausbauch
"missing" RNA Klaus Salger
Ligation problems Klaus Salger
DTT in in vitro transcription Rx jie li
Exo-Meth Sequencing Klaus Salger
software to generate restrcition fragment graph nico at lmb1.rug.ac.be
metal grid for raft culture ynakao at kean.ucs.mun.ca
Microwave techniques? LEES at SASK.USASK.CA
Ligation problems Klaus.Matthaei at ANU.EDU.AU
transfection quality DNA- help apeel at maple.circa.ufl.edu
VNTR loci analysis using Genescan Garry P. Larson
Freezing Bacteria-Liquid N2 OK? Rafael Maldonado
Insect Phenoloxidase Lisa Neven
Kits for genomic DNA from blood (lymphocy Annienell
ANNOUNCE: NEW WEB SITE - World Health Net Eric Klien
immunofluorescence background Yasuhito Abe
M-13 Purification kits Annienell
Southern with small probe? mpage at molbiol.ox.ac.uk
Legal Polymerase?? HOWARD at MGRP.BGSM.WFU.EDU
VectorNTI Manuel Maria SIMON
RT-PCR using S1 Nuclease Martin D. Leach
PCR sux's Clinton Edwards
PCR sux's Clinton Edwards
Ligation problems John and Alex
Electroporation John Fox
DTT in in vitro transcription Rx Frances Hannan Zoology
high background slot blots Mike Rott
M-13 Purification kits Richard E. Showalter
PCR primer design Richard E. Showalter
DTT in in vitro transcription Rx John Fox
transfection quality DNA- help John Fox
Microwave techniques? Martin Kennedy
TA cloning ratio Edward Wang
What's wrong with my DNA sequencing template? Michel Leroux
Favorite bacterial RNA prep?? John Fox
immunofluorescence background U12201 at uicvm.uic.edu
dig-RNA synthesis hcorbett
need Ab for poly (ADP-ribose)polymerase Incheol Shin
alignment software Mr SMNN Faruque
An enzyme degrading dsDNA but not ssDNA Mr. J. Sasse
Unsubscribe Banstead at aol.com
human polyA DNA Anne Spang
Ligation problems "Alexander Kraev" bckraev at aeolus.ethz.ch
TA cloning ratio "Alexander Kraev" bckraev at aeolus.ethz.ch
sequence programs Eric C. Anderson
(none) U12201 at uicvm.uic.edu
Cholesterol-rich food for mice U12201 at uicvm.uic.edu
Ligation problems Klaus Salger
PCRs no longer working. HELP! Shahram Mori
antibody compatibility Shahram Mori
RT-PCR using S1 Nuclease Shahram Mori
Church for Northern blots? Glen Shearer
BNN132 strain Colin MacD
Formaldehyde RNA gels. Dr Saleem Mohammed
shake after electroporation? Qunfeng Dong
ANNOUNCE NEW WEB SITE: World Health Net Eric Klien
Analysis of 2D-gels with scanner and PC John M. Garrison
Triton X-114 Endotoxin Removal-S.O.S. Martin Sorich
alignment software Zophonias O. Jonsson
An enzyme degrading dsDNA but not ssDNA Paul Robert Fisher
UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ BIOSCI Administrator
address requested (fwd) LEES at SASK.USASK.CA
looking for address (fwd) LEES at SASK.USASK.CA
Curing E.coli plasmids Duncan Clark
Q about boiling miniprep sequencing. David Micklem
Software for oligo Thierry Berthe
Radioactive probes by PCR.
Ligation problems "Alexander Kraev" bckraev at aeolus.ethz.ch
Direct selection for GalE in E.coli Duncan Clark
Request for actin clone (?) Tracy Aquilla
shake after electroporation? Michael Cooley
M13 antibodies for phage display Schuster, David
Microwave techniques? Paul N Hengen
Exo-Meth Sequencing otunnela at ktl.fi
Microwave techniques? Steven Striepeke
Microwave techniques? Randall J. Cohrs
PCR sequencing problem Walt Lilly
CsCl preps for ABI sequencing reactions Eric C. Anderson
DNA sequence puzzle! aEscher at ccmail.llu.edu
shake after electroporation? kevin c. graham
TA cloning -Promega Mail at scri.sari.ac.uk
Ab for ferritin? Ojar Melefors
Desferrioxamine and E.coli. Ojar Melefors
Direct selection for GalE in E.coli Rafael Maldonado
magnetic bead question sl1nn at cc.usu.edu
Curing E.coli plasmids Rafael Maldonado
shake after electroporation? Rafael Maldonado
CAT assay problem! Klaus Salger
AIDS Research Brent Zanke
Temp. Gradient Gel Electrophoresis/GC clamps MARLENE SNYDER
DNA sequence puzzle! Tom Fagan
Formaldehyde RNA gels. Karl Fischer
Site-directed Mutagenesis Oligos Karl Fischer
ATCC e-mail ITABAJARA DA SILVA VAZ JUNIOR - 201
How long would it take to clone a gene from a plasmid? Pete Muriana
How long would it take to clone a gene from a plasmid? Pete Muriana
PCR primer design Julian Wood
GENE CLEAN biukovic at olimp.irb.hr
pGex-cryptic promoters in GST? Dale Lindsley
BAC, cloning, HELP! SYME,JENNIFER ALBERTA,MS
Yeast 2-Hybrid Seth Corey
ds- versus ss-DNA sequencing DREWES at MPASMB.DESY.DE
DTT in in vitro transcription Rx Matt Thomas
silver sequencing Eric W. Linton
Curing E.coli plasmids Tamas Gaal
Curing E.coli plasmids Tamas Gaal
Curing E.coli plasmids Tamas Gaal
protein kinase A inhibitors Jeff Rawlings
Why can't I get agarose embedded HMW DNA to digest ? Todd DeZwaan
HELP: Microtubuli or tubulin Todd DeZwaan
Site-directed Mutagenesis Oligos Todd DeZwaan
storing western blots Fiberman
CREB antibodies Fiberman
Silver Sequencing Eric W. Linton
ALTERNATIVE SPLICING marcvdc at lmb1.rug.ac.be
RT-PCR using S1 Nuclease Shahram Mori
Triton X-114 Endotoxin Removal-S.O.S. 2 Martin Sorich
Temperature gradient gel electrophoresis (TGGE) Shiao Y. Wang
Algae DNA Ambiocomm - Richard Dana
Want PCR to incorporate 32P label Shiao Y. Wang
Proteinase K Shiao Y. Wang
0.2-mL PCR tubes Shiao Y. Wang
suggestions on automated DNA sequencer? bcdlee at hkpucc.polyu.edu.hk
recombinant protein purification Dominique Schaller
Direct selection for GalE in E.coli Duncan Clark
TA cloning kit (In Vitrogen) Onno_Myriam
TA cloning kit (In Vitrogen) Onno_Myriam
Chelex Sophie Launey, These Andre Gerard 31 12 96, Ifremer DRV RA, 46.36.30.07
GFP: What is Coelenterazine thoenes
Adress of Tetko, Elmsford, NY derk at hubrecht.nl
Microwave techniques? John Dixon
CsCl preps for ABI sequencing reactions John Dixon
antisense oligonucleotides Jan Eggermont
An enzyme degrading dsDNA but not ssDNA John Dixon
serum and HeLa kreuzer at bkfug.kfunigraz.ac.at
CsCl preps for ABI sequencing reactions Anthony Otsuka
Marathon-RACE (clontech) inquiry Bradley Turner
Bio-Rad Phosphorimager h
Marathon-RACE (clontech) inquiry Bradley Turner
Running Retic lysate on SDS-PAGE h
CREB antibodies Martin D. Leach
ATCC e-mail Martin D. Leach
Transforming E. coli with Yeast DNA... HOW!? Esther Guarinos
Electroporation of Antibodies gettin' better
0.2-mL PCR tubes John Fox
ABI 373 and PCR pdt Lisa Bibbs
Getting out the brown from DNA Paul E. Ulanch
Site-directed Mutagenesis Oligos Emmanuel Skoufos, Ph.D.
mutanolysin Szatmari George
recombinant protein purification Zophonias O. Jonsson
CsCl preps for ABI sequencing reactions "Alexander Kraev" bckraev at aeolus.ethz.ch
AZTREONAM, HELP! Jack M. Bernstein, M.D.
RNA from Spleen - Help! Karl Fischer
help and advice on 5' RACE Robert Molday
Running Retic lysate on SDS-PAGE Darren Boehning
RNA from Spleen - Help! Aaron Pawlyk
RNA from Spleen - Help! Aaron Pawlyk
Fluorescent Proteins Newsgroup proposal clontech!paulki at NETCOM.COM
GeneClean vs Eluquik vs Prep-a-gene Skory
recombinant protein purification Mxm
Direct fluorescein labeling of DNA Edward Beaty
Storage of PEG solutions Liam E Good
antisense oligonucleotides DJC poland
Marathon-RACE (clontech) inquiry Jon Nakamoto
pLysS freezer stock instability Edward Beaty
Silver Sequencing U12201 at uicvm.uic.edu
storing western blots U12201 at uicvm.uic.edu
software to generate restrcition fragment graph John Hodgson
Journal Table of Contents GORDON BETTS
RNA from spleen ANDREI POPOV
PCRs no longer working. HELP! hodges bradley l
cytokine Response elements Alec Redwood
PKC assay wanted!! leon at hubrecht.nl
GENE EXPRESSION Soeren Jensby Nielsen
antisense oligonucleotides M K Bennett
E. coli W3110 not accepting two plasmids Ralf Morgenstern
Temp. Gradient Gel Electrophoresis/GC clamps M K Bennett
PCRs no longer working. HELP! "Alexander Kraev" bckraev at aeolus.ethz.ch
Temperature gradient gel electrophoresis (TGGE) M K Bennett
peptide expression in E.coli M.R.douglas at rheuma.bham.ac.uk
Algae DNA WAS
Swansoine willingmann at uke.uni-hamburg.de
HELP: pyrroletricarboxylic acid, thiazoledicarboxylic acid michael.micksche at univie.ac.atBettinaKammerer
What is the expression vector, pSG5? slee at opal.tufts.edu
Pischia expression H. David Fischer
Msp1 Genomic Digests DYDM6025 at iruccvax.ucc.ie
Church Buffer, high background DYDM6025 at iruccvax.ucc.ie
GENE EXPRESSION Martin D. Leach
Optimum temp for PCR primer annealing Allen Gathman
Postdoctoral Position Tien-Hsien Chang
storing western blots Gerard
Problems with densitometry!....for WESTERN BLOTS Gerard
Software for oligo dieter.deforce at rug.ac.be
ssDNA synthesis CVLowry
Msp1 Genomic Digests Duncan Clark
E. coli W3110 not accepting two plasmids Duncan Clark
in vitro transcription/ab-depletion Ojar Melefors
Optimum temp for PCR primer annealing Andy Law Big Nose
Antibodies against Thioredoxin? Richard Mandel
Enriching gene copy no. G
PCR primer help! Wayne Gottlieb
TA cloning kit (In Vitrogen) Wayne Gottlieb
PCRs no longer working. HELP! Dr Keith Grimaldi
Direct fluorescein labeling of DNA hanzel at mdyn.com
Journal Table of Contents Shaun D. Black
BbvII Restriction enzyme Paul Riggs
suppliers of Fse1,Sse83871, BspG1 ??? Paul Riggs
Direct fluorescein labeling of DNA U12201 at uicvm.uic.edu
Getting out the brown from DNA Robert Preston
Problems with densitometry!....for WESTERN BLOTS jfs at WH.BAYER.COM
DHFR and methotrexate Barry Schweitzer
Electrophoresis Help Essex Research
non-palindromic RE? Randall J. Cohrs
M13 antibodies for phage display williams at bscr.uga.edu
Running Retic lysate on SDS-PAGE John A. Newitt
DNA sequence puzzle! Paul N Hengen
storing western blots Paul N Hengen
Hemoglobin fluorescence Dave Bates
storing western blots Paul N Hengen
Large Scale Insect Cell Culture Gwen Shafer
Problems with densitometry!....for WESTERN BLOTS Paul D. Boyer
GFP in CHO cells Grtful ned
protein marker for insect microsomes Darren Fast
Subcloning large fragments Nick Ellison
Temp. Gradient Gel Electrophoresis/GC clamps molpath at chmeds.ac.nz
freq of amber revertants in lambda ZAP? John Dixon
Subcloning large fragments "Alexander Kraev" bckraev at aeolus.ethz.ch
GFP problems. Any tips? Grtful ned
Hemoglobin fluorescence David S. Gottfried
Taq-Pfu compatibility Tom Doak
M13 ssDNA degradation? Dubear Kroening
recombinant protein purification Dominique Schaller
PCR amplification of cloned insert Gil
UNSUBSCRIBE anantha r kolleivgode
PCRs no longer working. HELP! dieter.deforce at rug.ac.be
address requested (fwd) Eric C. Anderson
Journal Table of Contents U12201 at uicvm.uic.edu
PKC assay wanted!! U12201 at uicvm.uic.edu
Problems with densitometry!....for WESTERN BLOTS U12201 at uicvm.uic.edu
Help! RNA extraction Andrew Brooks
PCR amplification of cloned insert Paul N Hengen
Repeat PCR for mouse genomic DNA Markus Grompe, MD
Glycerol shock in transfection-mechanism? William H Ashman
HELP! Need enzyme (cyclophilin) fast J.P. Germanas
transcription inhibitors Jeff Kramer
Depurinate & Break DNA? Miguel Angel Rodriguez Gabriel
Hemoglobin fluorescence Dave Bates
-----------------UK----------------------- PN WRIGHT
/equipment/ABI380B DNA SYN. John Gardner
Sea Urchin Eggs: Fertilization and PI cycle MICHAEL R ACAB
Transiet transfection of neurons Matthew Frosch
electrophorisis of double nicked circle Andrew Belt
formaldehyde pH Harry Yim
protein expression on a contract basis bipin dalmia
Chemical Cleavage of Proteins/Peptides Angeline Kantola
promoter/intron-exon people: wanna help me?? Heidi Moss
WANTED: research associate in protein expression bipin dalmia
Exo-Meth Sequencing Janne Tornow
Qiagen 96-well maria at acme.llnl.gov
electrophorisis of double nicked circle HARDIES at THORIN.UTHSCSA.EDU
pSG5 form Stratagene! gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
electrophorisis of double nicked circle David Gonda
(none) Sherif Abouelela
Help! Unexplained deletion in gene Ramkumar Lachumanan
Optimum temp for PCR primer annealing mmg6
Subcloning large fragments Susini Laurent
s.moore
test Mohohlo Molatudi Botany
Optimum temp for PCR primer annealing Hui Yuk Fun
electroporator colleen at chempath.uct.ac.za
pcDNA3 Mick Jones
PROMEGA ??? Mike Morris
Mutagenesis : help! Mike Morris
farnesyl transferase (Piotr Kozlowski)
Periplasmic extract/His tagged Prot?? Mxm
BbvII Restriction enzyme Duncan Clark
Methods shortcourse? Allan Berger
MW of IgG? Tracy Aquilla
PROMEGA ??? Martin D. Leach
Help! RNA extraction Aaron Pawlyk
non-palindromic RE? Jillian Johnston
thrombin antibodies? Ding Ming
Problems with densitometry!....for WESTERN BLOTS Gerard J Madlambayan
Optimum temp for PCR primer annealing Allen Gathman
Problems with densitometry!....for WESTERN BLOTS Jeff Rawlings
Transfections using QIAGEN QIAGEN
unsubscribe KARN at Butler.EDU
farnesyl transferase John A. Newitt
microbiocides Hendrik Ballerstedt
Results: CsCl preps for ABI sequencing Eric C. Anderson
PCRs no longer working. HELP! Andre Hamel
Poser for PAGEophiles Rick Conrad
MW of IgG? Steve Konings
help and advice on 5' RACE Robin Reed
Attachment of primary cell cultures Fengling Chen
promoter/intron-exon people: wanna help me?? paiks at gunet.georgetown.edu
cosmid subcloning William Jia
Subcloning large fragments S.
in vitro transcription/translation Yasha Hartberg
Getting out the brown from DNA mpage at molbiol.ox.ac.uk
Electroporation of Protoplasts John Fox
Nicotiana "gene banks" LOGAND
LacZ Xsomal deletions Duncan Clark
Toothpick sources in the UK Duncan Clark
pcDNA3 Ian A. York
pcDNA3 Chris Silvia
Transfections using QIAGEN Martin D. Leach
Attachment of primary cell cultures Martin D. Leach
promoter/intron-exon people: wanna help me?? Martin D. Leach
Toothpick sources in the UK Martin D. Leach
Chemical Cleavage of Proteins/Peptides U12201 at uicvm.uic.edu
Problems with densitometry!....for WESTERN BLOTS T.S. Pillay
Problems with densitometry!....for WESTERN BLOTS T.S. Pillay
Expression library for MHC H-2Db skcbiol
Stratagene's Quik Hybridization solution
FAS(CD95) and FASL Jeffrey Upperman
Microwave techniques? Martin Kennedy
Toothpick sources in the UK Duncan Clark
Where to get Nonidet P-40 Karl Fischer
Toothpick sources in the UK Edward Beaty
Nonidet P-40 Lara Endres Baxley
Slowly migrating EGF receptor in SDS-PAGE Dayanidhi Raman
help and advice on 5' RACE Mikek
Cross-linking in proteins? ahillar at your-servername.Lan1.UManitoba.CA
Nonidet P-40 Chris Silvia
CLONING PCR PRODUCT Doug-young Ryu
pHC79, cosmid vector; supply Robert.Coelen at jcu.edu.au
re noniodet P40 Keith.Wright at WPO4.CL.HealthWaikato.HWL.synet.net.nz
(none) To: Nikolai
polystyrene biodegradation a
(none) U12201 at uicvm.uic.edu
MW of IgG? U12201 at uicvm.uic.edu
Formaldehyde RNA gels. Steven Sullivan
phosphoimager Fung Ming Chiu
Alternatives to Formalin/Formaldehyde? (Dave Johnston)
Toothpick sources in the UK Chris Boyd
Help! RNA extraction Unknown
Transfections using QIAGEN Amos Heckendorf
SPR independent translocation into ER Manuel Maria SIMON
German Merck contact Jennifer C. Quirk
Ghost bands on Western blot? Mr TG Downing
Where to get Nonidet P-40 ICAgen
Nonidet P-40 "Mayer, Jorge ", 3637
(none) Leonora Rochwerger
SURE vs. NovaBlue competent cells Malcolm Campbell
German Merck contact gustilo at pobox.upenn.edu
PCR Product Purification for Seq? Greg Gloor
MW of IgG? To: Nikolai
Antibodies against Thioredoxin? mccallo at randb.pprd.abbott.com
SURE vs. NovaBlue competent cells John Brunstein
in vitro transcription/translation Brick Ayola
Slowly migrating EGF receptor in SDS-PAGE John A. Newitt
Slowly migrating EGF receptor in SDS-PAGE John A. Newitt
Gentra Systems kits Charles M Griswold
Ghost bands on Western blot? Darren Fast
Qiagen 96-well QIAGEN
again polystyrene biodegradation Christoph Grunau
CLONING PCR PRODUCT Christoph Grunau
GST fusions Rob Steele
Toothpick sources in the UK daniel petersen
wierd cell culture contam C.J. Hussussian, MD
tissue culture cell membrane proteins Mark Farinha
DHFR substrates that can be crosslinked? Mark N. Mayhew
In situs in Drosoph. saliv. C. Chihara
test 190702082 Mcdonald, Ryan R.
cdna amplification Mr Vid Mohan-Ram
AD test posting a.dodd at auckland.ac.nz
molecular modeling software Gayle Christopher
LacZ Xsomal deletions Rafael Maldonado
Urea SDS-PAGE AMCKINNE at UTKVX.UTCC.UTK.EDU
Urea SDS-PAGE ANDREI POPOV
Urea SDS-PAGE Brian A. Hollander
Help!PCR screening protocol instead of miniprep Emma Croager
green fluorescent proteins Alec Redwood
Algae DNA Alec Redwood
RNA from Spleen - Help! Alec Redwood
oxidation of SS bridges Christopher Walentas Bioc
core mills corp Alec Redwood
Electroporation of Antibodies Dan Burgess
Sequencing Lambda DNA Rene.DeMot at agr.kuleuven.ac.be
ECL problems Graham Atherton
Gamma-Phosphate linked ATP-Sepharose (UBI) for purifying protein kinase DREWES at MPASMB.DESY.DE
Wanted - Murine IL-10 cDNA construct bwalker at comp.nibsc.ac.uk
CLONING PCR PRODUCT b-butler at nimr.mrc.ac.uk
Mitochondrial DNA inheritance Hector Cruz-Lopez
PCR Inhibition Water Chloramines Robert Preston
tissue culture cell membrane proteins George F Edick
cDNA library construction A van der Plas
Gentra Systems kits Scott Selby Med Heme
wierd cell culture contam Sean David Moore
Operational Amplifiers gvacano at eagle.wesleyan.edu
Random Peptide Libraries Workshop R. Wayne Litaker
mRNA half-life Leonora Rochwerger
yeast two-hybrid Seth Corey
unexplained insert from PCR clone? Steve Bryson
Fisher Scientific Duncan Clark
Methylmercuric hydroxide- SOURCE? Bradley Turner
in vitro transcription/translation Manuel Maria SIMON
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES Xin-Hua Zhou
ECL problems John A. Newitt
Electroporation of Antibodies brett at BORCIM.WUSTL.EDU
Source of thin walled tubes (U.K) Keith
Plasmid nomenclature MUNGO at UVVM.UVIC.CA
degenerate primers PCR David B. Stern
Differnetial Display Don Haut
Stratalinker : help needed stephan at lmb1.rug.ac.be
Source of thin walled tubes (U.K) Chris Jones
Thin-walled PCR tubes (UK) Keith
Where to get Nonidet P-40 Paul N Hengen
plasmid nomeclature "Mayer, Jorge ", 3637
IMPORTANT - UPDATE ON BIOSCI MAIL QUEUE PROBLEMS BIOSCI Administrator
Sequencing Lambda DNA jitu
C-terminal sequencing? Mandy Johnstone
Need human B cell expression library Carlos Moreno
Frequently Asked Question Pointer Paul N Hengen
Methylmercuric hydroxide- SOURCE? Tom Thatcher
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES John M. Freeman
Thiofusion expression system Emma Macfarlane
Fisher Scientific Shaun D. Black
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES Malcolm Campbell
GeneClean vs Eluquik vs Prep-a-gene R Daines
pcDNA3 R Daines
Vent DNA polymerase and asymmetric PCR Tim Buss
Source of thin walled tubes (U.K) (Dave Johnston)
oxidation of SS bridges Vennie
phosphoimager Manuel Maria SIMON
phosphoimager Fung Ming Chiu
tgf-beta Clemens Suter-Crazzolara
Help: I need the plasmid pGFP-N3 (distributed by Clontech) Werner Vogetseder
Microwave techniques? Mike Dalrymple
RNA from spleen Chris Hodgson
Toothpick sources in the UK U12201 at uicvm.uic.edu
formaldehyde pH David Glover
INValphaF' Juergen Sasse (Path)
PCRs no longer working. HELP! Dawn Gray
Chemical Cleavage of Proteins/Peptides Wen-Yen Kao
RT-PCR with Dynabeads Roger Besançon
troponin I a.buchan
Cross-linking in proteins? To: Nikolai
Idaho Thermocycler Todd Johnson
Phylogenetic tree construction Garry P. Larson
Where to get Nonidet P-40 Paul N Hengen
5' and 3' end of cDNAs by PCR Bernd Stein
Procaryotic RNA isolation Daniel Solaiman
cdna pcr Mr Vid Mohan-Ram
Wax beads Jody k. Hirsh
Vent DNA polymerase and assymmetric PCR molpath at chmeds.ac.nz
antisense oligonucleotides Gavin Fischer
Transfection of human primary fibroblasts eric at ericpc.mni.mcgill.ca
Nonidet P-40 U12201 at uicvm.uic.edu
green fluorescent proteins William J. Buikema
PCR CLONING AND FASLE WHITE COLONIES Christopher Hoover
FAS(CD95) and FASL Grtful ned
RUBISCO ANTIBODIES agpcrv at palm.cri.nz
Invitrogen SP6 and T7 primers... E. J. Meharra
LOW EFFICIENCY OF PCR CLONING AND FASLE WHITE COLONIES a.dodd at auckland.ac.nz
Differnetial Display zinetti at acpub.duke.edu
Lac repressor-B-gal fusion protein? Greg Arnold
YAC fingerprinting Simon Foote
Cryptic splicing of mammalian cDNA in transfected TC cells Harry_Au at SOM-LRC.UCSD.EDU
Mosaicism in X. laevis embryos anmab2 at orion.alaska.edu
pSV3-neo Steven Loechel
CLONING PCR PRODUCT Loryn Sellner
Trouble with Fisher brand cartridge for Nanopure Donald Chun Kit Wong
4-aminobutyric acid Mohohlo Molatudi Botany
need hsp70 Matthias Zeiner
Need help on RNA isolaton from plant Soo-Hwan Kim
protein/polysach contaminants M.DELAVINA at CGNET.COM
GAL4 Antibodies? Dr. S.L. Mathias
TA cloning : clone contamination chukins
Wax beads RandBio
Cross-linking in proteins? U12201 at uicvm.uic.edu
MW of IgG? Jack M. Bernstein, M.D.
Nonidet P-40 U12201 at uicvm.uic.edu
Biotinylation of bradykinin Edwin Fink
best immunogen, injctn. schedule for rabbit Paul A Bucciaglia
Help on PCR using modified DNA bases guru at STUBBE-LAB.MIT.EDU
GAL4 Antibodies? Konstantine Sarafis
phosphoimager Tracy Aquilla
NIH Image Dr. Robert Bateman
Mouse cDNA for EGFR croyleml at ucbeh.san.uc.edu
Again! help for crosslinking proteins to DNA ? stephan at lmb1.rug.ac.be
TG repeats (microsatellites) G Gallagher
Disulfide bonds Francesca Bahr
Invitrogen SP6 and T7 primers... John Dixon
Northern with non-radioactively labelled probe Muhammad Lodhi
differential display Richard R. Hardy
test cynthia stryker
Need DNA method for algae Ambiocom - Richard Dana
Bal31 source John Brunstein
Phosphorimager Questions John Brunstein
need help on RNA isolation from plant Sharon Regan
(none) Michael Johns
Help: I need a plasmid: pGFP-N3 (green fluorescent protein) Werner Vogetseder
Protein-DNA Crosslinks Scott A. Ness
buying a thermocycler anne at lmb1.rug.ac.be
differential display anne at lmb1.rug.ac.be
TA cloning : clone contamination txpljfg at uabcvsr.seas.ucla.edu
Trouble with Fisher brand cartridge for Nanopure JAG at QUCDN.QueensU.CA
UK supplier of Seaplaque GTG LMP agarose Simon Baker
need hsp70 Sandy Curtiss
core mills corp Martin D. Leach
test cynthia stryker
test cynthia stryker
test, ignore cynthia stryker
CsCl vs column kits cynthia stryker
Any one used Oligos from Universal DNA Inc? $1/base! PRAKASH at ACD.TUSK.EDU
Protein-DNA Crosslinks Chuck Miller
Enzyme-chimeras FNINER
Nucleic acid/protein sequence analysis software for Mac FNINER
apoptosis detection method SHO1
CsCl vs column kits Sean David Moore
Calcium removal from Calmodulin Gabriel Akopian
AB1899 phenotype shua!
Smiling sequencing gels Daniel Mcgoldrick
Any one used Oligos from Universal DNA Inc? $1/base! Michael Kloth
PCRs no longer working. HELP! Jim Gore
Suggestions for increasing phage stability? Kevin Lease
GCG manual Doug-young Ryu
address of Molecular Probes Inc. ?? Dr.
Stain for Copper Proteins marius brouwer
Testing... Do not read. jerian
harvesting bone cells from ceramics POSTE 4
How do you calculate annealling temperature if DMSO added??? Bixing Huang
Suggestions for increasing phage stability? Brad Nicholson
address of Molecular Probes Inc. ?? Bradley Turner
Differential Display Olivier Gandrillon
[Q]Antibody database Koichi Ozaki
Northern Red Shrimp/Pandalus borealis Duncan Clark
NIH Image Duncan Clark
Sokolov/Prockop DDRT Olivier Gandrillon
Protocol for downward blot lmjakt at hkucc.hku.hk
address of Molecular Probes Inc. ?? Tom Maynard
Invitrogen SP6 and T7 primers... John Dixon
address of Molecular Probes Inc. ?? BANANA & PLANTAIN
long range PCR Keith
DNA-Protein interactions Handbook Ralf Kleyer
Help: I need a plasmid: pGFP-N3 (green fluorescent protein) Frances Hannan Zoology
DNA-Protein interactions Handbook Georg Kroeger
Bcl-X antibody Graham Atherton
CsCl vs column kits Ian A. York
Fluorescein-11-dUTP M.P.Woodland
M13 ssDNA stability Dubear Kroening
GCG manual Ding Ming
(none) Sergei Krasnyanski
Alternatives to Formalin/Formaldehyde? Stephen R. Lasky, Ph.D.
CsCl vs column kits Bob Kodrzycki
CsCl vs column kits Emmanuel Skoufos, Ph.D.
96-well microtiter plates maria at acme.llnl.gov
ABI 373 and PCR pdt Eric C. Anderson
mRNA in situ on sections with DIG-probe? Katarina Nordqvist
Ligation of large plasmids Bob Kodrzycki
Gentra Systems kits Ian A. Boussy
biotin background problem Diogo Cavaco
green fluorescent proteins Stuart Kuhstoss
Thioredoxin fusion expression system Kathryn Graham
Vent DNA polymerase and assymmetric PCR Robert Horton
CsCl vs column kits Peter Myler
Whatman Filter Paper Aaron Polesky
CsCl vs column kits Andy Kravetz
GCG manual Robert Horton
in vitro transcription/ab-depletion hcavaco at fm.ul.pt
(none) Sergei Krasnyanski
y Rika Vanhuizen
degenerate primers PCR Steven Goldberg
DNA sequencing puzzle Alan
Wax beads G Shafer
Protocol for downward blot Paul N Hengen
Proposed FDA Antibody Regs May Affect You G Shafer
wierd cell culture contam Jason Kantor
GAL4 Antibodies? Don Jackson
polyclonal anti-mouse-hsp90 wanted ghilardi at ubaclu.unibas.ch
Labelling proteins in plant protoplasts c515455 at mizzou1.missouri.edu
address of Molecular Probes Inc. ?? Parke K. Flick
alfalfa mosaic virus enhancer? Bill
IMPORTANT - BIOSCI MAIL QUEUE BACK TO NORMAL BIOSCI Administrator
5'-RACE PCR for missing cDNA ends? DREWES at MPASMB.DESY.DE
Nonidet P-40 Steve Danko
y Bill
RT-PCR with Dynabeads jm
buying a thermocycler jm
Reference on urea breakdown John Altman
Wax beads Robert Horton
mRNA in situ on sections with DIG-probe? Christoph Grunau
y Rika Vanhuizen
y Rika Vanhuizen

Last message date: Fri Mar 31 23:41:52 EST 1995
Archived on: Sun Mar 6 20:29:57 EST 2005

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