E. Coli strain carrying T7 RNA pol ?

Chris Yates C.J.D.Yates at bham.ac.uk
Mon May 1 18:36:42 EST 1995


In article <Pine.ULT.3.90a.950430180920.16490B-100000 at bgumail.bgu.ac.il> aflaloc at BGUMAIL.BGU.AC.IL (Claude Aflalo) writes:
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>From: aflaloc at BGUMAIL.BGU.AC.IL (Claude Aflalo)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: Re: E. Coli strain carrying T7 RNA pol ?
>Date: 30 Apr 1995 08:11:14 -0700
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>On Sun, 30 Apr 1995, mahmoud wrote:

>> Hi 
>> 
>>       I was wondering is there a strain of E. Coli carrying T7 RNA POL
>available 
>> commercially?
>> 
>> 
>> Thanks in advance
>> 
>> mahmoud 
>> 
>Yes, BL21 

well as I understand it, you must have a strain (BL21 is good for protein 
expression as it is lon- and ompT-) which is lysogenic for lambda DE3. Lambda 
DE3 carries the T7 RNA polymerase gene under lacUV5 control.  BL21(DE3) is 
avaliable from Novagen.
          Chris.






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