Ferret Brain cDNA Library Mystery

ANDREI POPOV ANDREI.POPOV at bbsrc.ac.uk
Mon May 1 04:38:21 EST 1995


>Anyway, as said before, secondary structure can be a real problem.  If you
>try to make a new library, you might consider a reverse transcriptase with
>increased thermostability.  I hear that Tth polymerase has RT activity at
>70C.  Good luck. 

Tth does have RT activity but it is much, much weaker than
that of traditional r.transcriptases. It might be enough for
RT-PCR, but you ll need a kilogramm of Tth for cDNA libarary.

just a moan from a person who killed some time on Tth




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