Yeast as an alternative expression system
Sin Wun Chey
mcbsinwc at LEONIS.NUS.SG
Mon May 1 20:03:28 EST 1995
I have a big protein to express (aprrox. 150kDa). The E. coli GST
expression system is prone to breakdown of large proteins and has the
disadvantage of not being post-translationally modified. Have
anyone tried the GST-expression system in yeast? It seems to combine the
benefits of a quickie job (unlike baculovirus) and the production of
a translationally modified protein.
Any suggestions welcome!
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