Yeast as an alternative expression system

Sin Wun Chey mcbsinwc at LEONIS.NUS.SG
Mon May 1 20:03:28 EST 1995


Dear netters,
I have a big protein to express (aprrox. 150kDa). The E. coli GST 
expression system is prone to breakdown of large proteins and has the 
disadvantage of not being post-translationally modified. Have 
anyone tried the GST-expression system in yeast? It seems to combine the 
benefits of a quickie job (unlike baculovirus) and the production of 
a translationally modified protein.
Any suggestions welcome!

Thanks,
Wunchey
 



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