HELP! My PCR stop working!!!!

[Kevin G. Korth]

On your list of things you tried, I didn't see any mention of water 
quality.  It is possible that DNases in your resuspension water have 
destroyed your primers (yes, there are such a thing as ssDNases).  It is 
recommended to filter sterilize AND autoclave AND UV irradiate water used 
in the PCR process and reagents used in the PCR process.

Sorry, you may have to re-order those primers.  Have you tried running 
them on a high concentration gel to make sure they are still the right 
size?