DIG oligo 5'-end labeling

Tracey Forster ag296 at FreeNet.Carleton.CA
Tue May 2 09:57:39 EST 1995


I currently use 32-P to end label my oligo rRNA probes
(17-21-mers). Because I required quantification this was the
easy and obvious route.  I now have a need to use these probes
for routine detection from colony lifts and I was considering
using DIG labeling.  BMC has a kit for end labeling
amino-substituted oligos using digoxigen-NHS-ester, has anyone
tried it?  Does it give acceptable results? any drawbacks?

Bob Forster
Centre for Food and Animal Research
Ag Canada
Ottawa, ON



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