A: PCR not working

Susan Mango smango at macc.wisc.edu
Wed May 3 13:32:48 EST 1995


I've heard that polymerases with exo activity tend to eat up the
primers. therefore you get the best of all possible worlds if you mix
taq with pfu or taq extender. bigger yields and better accuracy. i've
mixed the two and it seemed to work well, but i've never sequenced my
fragments. 
good luck, susan
UW-Madison
smango at macc.wisc.edu



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