HELP! Establishing number of target molecules

wick at cris.com wick at cris.com
Wed May 3 15:48:45 EST 1995


I am interested in establishing the sensitivity of my DNA amplification reaction by determining the specific number of original  targets 
from which I can detect final product. How do I best determine the number of target molecules put into my amplifcation 
reactions---OD ? Fluorometry? Your suggestions would be greatly appreciated.

Jim Wick

E-mail:		wick at cris.com		
Phone:		414-871-7199
FAX:		414-871-1273
Snail Mail:	5520 W. Burleigh St.
		Milwaukee, WI 53210

"Don't precipitate more than you can resuspend"






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