RT-PCR

Ramkumar Lachumanan medp4033 at nusunix2.nus.sg
Thu May 4 04:50:11 EST 1995


Hi netters,
        I'm having problems with my RT-PCR....think I have internal 
deletions in the cDNA due to secondary structures during RT-PCR. So, do 
any of you guys out there have any information on a RT-PCR kit that works 
at elevated temperatures i.e has a thermostable rev. transcriptase? Am 
pretty desperate. All help is welcomed!!!!! Thanks.
                                                    Ram(ses) 
  




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