Two-hybrid problem...possibly

Simon Dawson mbxspd at
Thu May 4 04:31:45 EST 1995

	Has anybody successfully used the vector, pAS2 in the 2-hybrid system? 
Is it really much better than pGBT9? The reason for asking is we seem to 
be having immense problems actually cloning into pAS2, whereas pGBT9 
seems to be no problem at all. Seems a bit strange to me. Has anybody 
got any ideas as to why this might be?
	We normally transform the initial constructs into DH5alpha cells to 
screen for insert containing clones etc. Is there a problem with these 
cells perhaps?
	Any suggestions would be greatly appreciated as it's driving us mad.


Simon Dawson					TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry		FAX:+44 (0)115 9422225
Queens Medical Centre		Email:mbxpsd at
Clifton Boulevard				URL:
U.K.								"Back off man, I'm a scientist!"  -- Bill Murray.

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