mbxspd at unicorn.nott.ac.uk
Thu May 4 04:31:45 EST 1995
Has anybody successfully used the vector, pAS2 in the 2-hybrid system?
Is it really much better than pGBT9? The reason for asking is we seem to
be having immense problems actually cloning into pAS2, whereas pGBT9
seems to be no problem at all. Seems a bit strange to me. Has anybody
got any ideas as to why this might be?
We normally transform the initial constructs into DH5alpha cells to
screen for insert containing clones etc. Is there a problem with these
Any suggestions would be greatly appreciated as it's driving us mad.
Simon Dawson TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:mbxpsd at unicorn.nott.ac.uk
Clifton Boulevard URL: http://unicorn.nott.ac.uk/~mbxspd/Simon.html
U.K. "Back off man, I'm a scientist!" -- Bill Murray.
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