joe1 at onramp.net
Fri May 5 17:09:56 EST 1995
In article <3oa80j$7f3 at nuscc.nus.sg>, medp4033 at nusunix2.nus.sg (Ramkumar
> Hi netters,
> I'm having problems with my RT-PCR....think I have internal
> deletions in the cDNA due to secondary structures during RT-PCR. So, do
> any of you guys out there have any information on a RT-PCR kit that works
> at elevated temperatures i.e has a thermostable rev. transcriptase? Am
> pretty desperate. All help is welcomed!!!!! Thanks.
> I have been using SuperScript II preamplification kit from Life
Technologies(Gibco/BRL). The SuperScript II reverse transcriptase is RNase
H negative so you tend to get longer 1st strand synthesis. I then use
their Taq polymerase (has to be ordered separately). The same buffer is
used for both RT and the PCR.
Dallas VA Med Ctr/UTSWMC
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