molbio.methdss-reagnts

Andrew Hopkins hopkins at post.its.mcw.edu
Sat May 6 20:32:14 EST 1995


Does anyone know of a reliable method for detection of biotin labelled DNA
probes in southerns using tetramethylbenzidine (TMB) and streptavidin
horseradish peroxidase?  I have tried the method described by Kriegler in
"Gene Expression" which is copied from Helmuth in "PCR Protocols", but I
cannot get it to detect my probe.




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