What's pKONeo?

Marianna Max drmax at casbah.acns.nwu.edu
Sun May 7 21:28:51 EST 1995

In article <3oe33u$s7b at cisunix1.dfci.harvard.edu>,
Ian A. York <york at mbcrr.dfci.harvard.edu> wrote:
>Hidden in the old boxes in our lab in a plasmid called pKONeo.  Legend 
>has it that it's very good at making stable G418-resistant lines; but 
>nobody here now knows what it is.  In fact nobody here now has ever used 
>it, as far as I can tell.  Trying Genbank and vector databases didn't 
>turn up anything.  
>Anyone know what it is?  Who makes/made it?  Whether it actually is any
>good at making G418-resistant colonies? 

Well I have no idea what this plasmid might be but KO is suggestive of some
sort of knockout vector. Anyone in your group ever made a knockout mouse? 


>Ian York   (york at mbcrr.harvard.edu)
>Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
>Phone (617)-632-3921     Fax  (617)-632-2627

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