drmax at casbah.acns.nwu.edu
Sun May 7 21:28:51 EST 1995
In article <3oe33u$s7b at cisunix1.dfci.harvard.edu>,
Ian A. York <york at mbcrr.dfci.harvard.edu> wrote:
>Hidden in the old boxes in our lab in a plasmid called pKONeo. Legend
>has it that it's very good at making stable G418-resistant lines; but
>nobody here now knows what it is. In fact nobody here now has ever used
>it, as far as I can tell. Trying Genbank and vector databases didn't
>turn up anything.
>Anyone know what it is? Who makes/made it? Whether it actually is any
>good at making G418-resistant colonies?
Well I have no idea what this plasmid might be but KO is suggestive of some
sort of knockout vector. Anyone in your group ever made a knockout mouse?
>Ian York (york at mbcrr.harvard.edu)
>Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
>Phone (617)-632-3921 Fax (617)-632-2627
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