incorporating dye labelled nucleotide into PCR product
jspencer at blackburn.med.su.oz.au
jspencer at blackburn.med.su.oz.au
Sat May 6 00:24:51 EST 1995
Help! I need some advice about incorporating a dye labelled nucleotide into a
PCR product. Am using Molecular probes BODIPY-TMRX-14-dUTP.
Questions:-
1.What percentage of dyelabelled nucleotide to incorporate?
2.Should I decrease the level of dNTP, or just add the dye-labelled nucleotide
to the standard mix of ATCG?
3.Does the dye label affect cutting with restriction enzymes?
4.How can I remove the unincorporated dUTP
thanks, Jenean
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