insertion of DNA into E. coli chromosome

[Brad Nicholson]

In article <elsemoda-0805951142520001 at elsemore.es.dupont.com>, 
elsemoda at esvax.dnet.dupont.com (David A. Elsemore) wrote:

> Hi,
 >
 >I need to insert a 7.5 kbp fragment of DNA into the E. coli chromosome.
>snip<

Dr. Kenneth Timmis is probably your best bet. He has produced a series of 
mini-Tn5 based constructs.  
See, De Lorenzo, Herrero, Jakubzik & Timmis. 1990. MIni-Tn5 Transposon 
Derivatives for Insertion Mutagenesis, Promoter Probing, and Chromosomal 
Insertion of Cloned DNO in Gram-Negative Eubacteria. Journal of 
Bacteriology. 172:6568-6572.

Brad
 
===========================================================================
Brad Nicholson              |"If it worked the first time, it wouldn't be 
Department of Pathology     |	research."...Brad Nicholson
University of Utah          |	Live from behind the Zion Curtian.
Salt Lake City, UT 84132    | 
brad at corona.med.utah.edu    |
or: (801)-581-4365          | My opinions are solely my own.
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