insertion of DNA into E. coli chromosome
brad at corona.med.utah.edu
Mon May 8 19:01:06 EST 1995
In article <elsemoda-0805951142520001 at elsemore.es.dupont.com>,
elsemoda at esvax.dnet.dupont.com (David A. Elsemore) wrote:
>I need to insert a 7.5 kbp fragment of DNA into the E. coli chromosome.
Dr. Kenneth Timmis is probably your best bet. He has produced a series of
mini-Tn5 based constructs.
See, De Lorenzo, Herrero, Jakubzik & Timmis. 1990. MIni-Tn5 Transposon
Derivatives for Insertion Mutagenesis, Promoter Probing, and Chromosomal
Insertion of Cloned DNO in Gram-Negative Eubacteria. Journal of
Brad Nicholson |"If it worked the first time, it wouldn't be
Department of Pathology | research."...Brad Nicholson
University of Utah | Live from behind the Zion Curtian.
Salt Lake City, UT 84132 |
brad at corona.med.utah.edu |
or: (801)-581-4365 | My opinions are solely my own.
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