Problem with making large PCR fragments with TAQPlus

Karl Lcking klucking at aki.ku.dk
Tue May 9 06:38:10 EST 1995


Hello.

We have tried Stratagenes TaqPlus for making large PCR fragments but it 
seems to work rather inconsistently. Sometimes - sometimes not!
They write in a sheet that they use 1 microli. of a 100 mM dNTP stock in a 
reaction vol. of 100 microlitre, - could that be correct.

Anyone with experience - give us a hint.

karl.



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