Problem with making large PCR fragments with TAQPlus

Sailesh Surapureddi SaiSu at MCB.LiU.SE
Tue May 9 17:58:51 EST 1995

>We have tried Stratagenes TaqPlus for making large PCR fragments but it 
>seems to work rather inconsistently. Sometimes - sometimes not!
>They write in a sheet that they use 1 microli. of a 100 mM dNTP stock in a 
>reaction vol. of 100 microlitre, - could that be correct.
>Anyone with experience - give us a hint.

Yes it is correct with reference to the dNTP conc. Try avoiding air bubbles 
while pipetting.
all the best. 
"i don't know what caffeine does for you, but i'm pretty sure that without it your head caves in."

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