cleared lysate prep
paolo de marco
lsraj at csv.warwick.ac.uk
Tue May 9 14:04:04 EST 1995
hi everybody !!
quick question: I want to quote th 'clear lysate' plasmid prep method
in my thesis, but I have only the method written down with no reference and I
can't remember who or where from I got it ?
does it ring a bell to anybody ??
can you kindly point me out to a reference ?
the prep is like so :
- lysozime lysis in Tris/sucrose
- +EDTA+ Triton-X-based mix
- precipitation of DNA in cold PEG/NaCl
- CsCl grad
thanks a lot in advance fro your help
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