cleared lysate prep

paolo de marco lsraj at
Tue May 9 14:04:04 EST 1995

hi everybody !!
    quick question: I want to quote th 'clear lysate' plasmid prep method
in my thesis, but I have only the method written down with no reference and I
can't remember who or where from I got it ?

does it ring a bell to anybody ??
can you kindly point me out to a reference ?

the prep is like so :

- lysozime lysis in Tris/sucrose
- +EDTA+ Triton-X-based mix
- spin
- precipitation of DNA in cold PEG/NaCl
- CsCl grad

thanks a lot in advance fro your help 

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