Need Glu/Asp-modification at pH7
macht at chclu.chemie.uni-konstanz.de
Tue May 9 10:23:18 EST 1995
Does anyone know a method for modification of Asp/Glu-sidechains under neutral
conditions (pH 6-7.5) in salt buffers at 37 degree (reaction time doesn't
really care) ?
I want to perform a peptide-sidechain modification of a peptide bound to an
monoclonal antibody while leaving the antigen-antibody bond intact. In our
group there were tried modifications with carbodiimides, but they seemed to
work specific only at pH around 4.5. The modification should deliver a
recognizable mass shift (i think more then 20 amu will do it) for mass
spectrometric identification of the modifcated residues.
If you know a suitable method for this reaction please mail it to me
(macht at chclu.chemie.uni-konstanz.de) or post it. Yours sincerly
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