cleared lysate prep

Sailesh Surapureddi SaiSu at MCB.LiU.SE
Tue May 9 18:08:18 EST 1995

>hi everybody !!
>    quick question: I want to quote th 'clear lysate' plasmid prep method
>in my thesis, but I have only the method written down with no reference and I
>can't remember who or where from I got it ?
>does it ring a bell to anybody ??
>can you kindly point me out to a reference ?
>the prep is like so :
>- lysozime lysis in Tris/sucrose
>- +EDTA+ Triton-X-based mix
>- spin
>- precipitation of DNA in cold PEG/NaCl
>- CsCl grad
>thanks a lot in advance fro your help 
>					paolo

You can find the reference in maniatis page 1.105 and 1.107, the names are 
Birnboim et al (1979) and Ish-horowicz et al (1981).
The articles are quoted on page 1.25 of the same volume.
"i don't know what caffeine does for you, but i'm pretty sure that without it your head caves in."

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