Help! pfu DNA polymerase for PCR
John P. Aris, Ph.D.
johnaris at anatomy.med.ufl.edu
Wed May 10 04:35:38 EST 1995
I have been using the pfu DNA polymerase to try to amplify a 3-kb fragment, I
tried different conditions such as annealing temperatures at 45, 50, 55, 60
degrees, extension time 6, 7 and 8 minutes at 72 degrees. I am using the yeast
genomic DNA as template, I tried 10 and 100ng per 50 ul reaction and I also
tried 2.5 and 5 units per 50 ul reaction, each time I used taq polymerase as
a control. I can obtain a 3-kb product from taq but nothing from pfu. If
anybody knows how pfu works, would you please tell me about it? Thanks.
Anatomy and Cell Biology
University of Florida
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