HELP! Genomic DNA not cutting!

galinm01 at mcrcr6.med.nyu.edu galinm01 at mcrcr6.med.nyu.edu
Thu May 11 18:19:03 EST 1995


Hello!

Seeking suggestions on "how to" "clean-up"  genomic DNA that
refuses to digest with restriction enzymes.  Repeated phenol/chloroform
extractions have not helped. 

This DNA sample is semi-precious.  Will be grateful for advice and comments.

This problem has not been encountered before in this lab.
Numerous other preps of genomic DNA have been made with the labs 
standard isolation procedure, and have digested just fine.

Many thanks,
Mary






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