HELP! Genomic DNA not cutting!

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Fri May 12 13:05:00 EST 1995


Have you checked out Pierce Chemicals Xtreme?

In article <1995May11.191903.7840 at mcrcr6>, galinm01 at mcrcr6.med.nyu.edu says:
>Hello!
>
>Seeking suggestions on "how to" "clean-up"  genomic DNA that
>refuses to digest with restriction enzymes.  Repeated phenol/chloroform
>extractions have not helped.
>
>This DNA sample is semi-precious.  Will be grateful for advice and comments.
>
>This problem has not been encountered before in this lab.
>Numerous other preps of genomic DNA have been made with the labs
>standard isolation procedure, and have digested just fine.
>
>Many thanks,
>Mary
>
>
>



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