Frameshift mutation in pBS KS

ANDREI POPOV ANDREI.POPOV at bbsrc.ac.uk
Fri May 12 12:20:28 EST 1995


Hi
This mutation was documented in Sikorski at al
1989 Genetics 122:19-27 (Addendum)
The curious thing is that LacZ complementation
still works though people have noticed long ago
that cells with pBS vectors turn blue slower than
with pUCs.
I wonder how this mutation got there, maybe problems with C/G sequencing
are not purely in vitro artefacts? Maybe DNA polymerases
have the same problems in vivo?

Andrei




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