non-isotopic PCR primers?

Tay Hou Ngee Agnes mcbtayhn at leonis.nus.sg
Fri May 12 00:30:21 EST 1995


Hi
Does anyone have any experience with non-isotopic labels for PCR primers 
to detect dinucleotide polymorphism? I have done endlabeling with 32P and 
it works well but I'd like to switch to non-isotopic methods. Does 
biotniylation work? has anyone used agarose gels instead of PAGE for 
resolution of dinucleotide repeats?
Thanks 
Agnes




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