ECL detection kit
harold.drabkin at channel1.com
Sun May 14 01:16:00 EST 1995
Subject: ECL detection kit
E>figured out a way to stretch the life of
E>Amersham's ECL detection kit. I find that we have plenty of labelling
E>reagent, blocking reagent and somewhat less of the hybridization
E>solution, but we quickly burn through the detection reagent.
E>Does anyone know if you can either dilute the detection reagents, or even
E>better, what's in them so I can make my own?
E>Also, if you know what's in the hybridization solution, I'd like to know
E>that. I personally suspect that it's just SSC plus some gold color.
E>UNiversity of California, Davis
E>bcmeyers at ucdavis.edu
Don't know about the nucleic acid dectection kit, but I've used the
Western kit and have found that I can dilute the reagents 1:1 with
water, thereby doubling the number of blots that I can do.
This was actually suggested by the Amersham reps.
hdrabkin at mit.edu
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