smori at nmsu.edu
Sun May 14 11:14:40 EST 1995
schroeder at vzdmza.zdv.uni-mainz.de wrote:
: Dear netters,
: our problem is a dramatic reduction in transformation efficiency
: with several bacterial strains. Ligations, when analyzed on a gel,
: seemed to work fine. When used for transformation only very few colonies
: are growing, which are very small and grow very slowly. Usually these don´t
: contain inserts.
: We have changed all components we could think of but couldn´t see any improvement.
: Any suggestions?
: Any kind of help is higly appreciated
: Christian Schröder
Could the gene be lethal to the bacteria? If so you need to regulate the
expression of the gene by using other expression vectors.
C A N A D A
Program in Molecular Biology
Department of Chemistry and Biochemistry
New Mexico State University
Las Cruces NM
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