supercoiled DNA and transfection

shadow shadow at medicine.medsch.ucla.edu
Sun May 14 23:04:34 EST 1995


It is often said that plasmid DNA should be predominantly
supercoiled for it to be expressed efficiently following 
transfection.  Does anybody know of any published data on this?
Also, in my hands, the apparent amount of the supercoiled DNA 
can vary from one agarose gel to the next without any change in
transfection efficiency. Have other people noticed that, too?
This makes it difficult to draw any conclusions about the relation-
ship between supercoiling and transfection efficiency.



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