quantitative immunoassays

[Fiberman]

Hello, netter,

I wish to detect changes in the level of a specific protein expressed in
brain tissue.  I have an antibody that will recognize the protein.  What is
the best assay I can run to quantitate the change in expression?  I don't need
absolute numbers, but I need an assay that will accurately detect changes in
protein levels.  I have tried western blots, but I feel this assay has a lot
of room for variation.  I would appreciate any suggestions.

-fm