E.Coli prep

Glibert Fabrice glibert at ceph.cephb.fr
Mon May 15 11:17:49 EST 1995



hi all dear netters...

it's a new technique for me : DNA extraction
from bacteria...my method is good i think
but i have just a little problem : final
extract is very very viscous and it's a
bad thing for me...how can i have something
more watery, more thin ?
At this time i use lysosyme then NaOH preci
pitation...someone tell me to add proteinase K
between lysosyme and NaOH...i want something
very simple...may you help me ?
thanx in advance and...sorry for my english !

fabrice



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