More GFP info needed
gr8bn at cc.usu.edu
Sat May 13 12:48:16 EST 1995
In article <3p06d8$2cb8 at news.doit.wisc.edu> dubear at offroad (Dubear Kroening) writes:
>Relay-Version: ANU News - V6.1 08/24/93 VAX/VMS V6.1; site cc.usu.edu
>Subject: More GFP info needed
>Message-ID: <3p06d8$2cb8 at news.doit.wisc.edu>
>From: dubear at offroad (Dubear Kroening)
>Date: 12 May 1995 17:37:44 GMT
>References: <01HQEWVUL58I8WWFNX at VAX.CS.HSCSYR.EDU>
>Organization: University of Wisconsin, Madison
>Stefan Baeckstroem replied with information concerning GFP, but I
>could not reach him using e-mail.
>He suggested using agar plates with a dark color. Does anyone know
>what to add to achieve this?
>Also, he said not to use plastic petri dishes (I assume). Has anyone
>used glass petri dishes, are they reusable, and where does one obtain
>Thanks for any and all info concerning GFP.
>dubear at whitewater.chem.wisc.edu
We have a undergrade in our lab working on the GFP protein for his
undergrade research project. He used plastic petri dishes to grow the Ecoli
barring the pGFP plasmid at 37 C over night with or without IPTG. He put a
piece of black paper under the plates and shined with long wavelength UV.
It was quite clear to distinguish the Ecoli barring pGFP from negative
Gr8bn at cc.usu.edu
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