RNazol/lung RNA degradation

Tim Cullingford tculling at ion.bpmf.ac.uk
Mon May 15 09:46:05 EST 1995


I use RNazol (4M GITC/sodium acetate) for extracting brain RNA and it
works fine. However, liver and lung preparations end up with a degraded
smear on formaldehyde gel. I vaguely remember someone posting that one
could increase the GITC to 5M and liver RNA is OK. Could someone confirm
or instruct otherwise, best wishes 
Tim Cullingford



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