coli promoter probe
brad at corona.med.utah.edu
Tue May 16 18:44:37 EST 1995
In article <15MAY199519410179 at portia.caltech.edu>, earn at portia.caltech.edu
(JUST ERIC) wrote:
> Does anybody have (or know who does) a promoter-probe vector for E.
Check out-> Simons, Houman & Kleckner. 1987. Improved single and multicopy
lac-based cloning vectors for protein and operon fusions. Gene. 53:85-96.
Really good vectors and system to move promoter-reporter to single copy (in
the chromosome as a lambda lysogen). This can get rid of nasty artifacts
due to multi-copy effects. The only limits are the choice of sites, BamHI,
SmaI (In some cases) & EcoRI. If your promoter is known, you can PCR the
region of interest with appropriate ends, pop it in and off you go.
Brad Nicholson |"If it worked the first time, it wouldn't be
Department of Pathology | research."...Brad Nicholson
University of Utah | Live from behind the Zion Curtian.
Salt Lake City, UT 84132 |
brad at corona.med.utah.edu |
or: (801)-581-4365 | My opinions are solely my own.
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