DNA Sequencing +wedge spacers

Hiranya Roychowdhury hroychow at NMSU.EDU
Tue May 16 16:30:49 EST 1995


On 16 May 1995 bckraev at wawona wrote:

> In article <3pafbe$qm3 at mserv1.dl.ac.uk>, LOGAND <logand at msdos.ensam.inra.fr> writes:
> >Dear All,
> >
> >Planning to use wedge spacers (0.2 - 0.75 mm) in place of uni-width spacers 
> >so as to increase the eveness of the separation between bands in my 
> >sequencing gels. I use the Bio-Rad Sequegen apparatus and their large gel 
> >dryer. My question is - do I need to change the drying program for this 
> >type of gel or does the standard program (with the initial peak then 
> >plateau over two hours) suffice? 
> 
> Wedge gels dry perfectly if they are covalently bound to one of the
> glass plates ( and you don't need the gel dryer ).
> I have used this for a long time, since our dept purchased Pharmacia's
> Macrophor devices. However, you'd better use "electrolyte gradient" 
> ( that is, 0.8-1 M sodium acetate in the lower tank ), or even better, t
> he LongRanger matrix. Both are much more straightforward ways of increasing 
> resolution than is the wedge gel.
> Hope this piece of advice comes not too late, it is 21:30 here...>
> *************************************************************************
> Alexander Kraev, Ph.D.                 Internet: bckraev at aeolus.ethz.ch
> Lab. of Biochemistry III               Phone: 0041-1-632-31-47
> Swiss Federal Institute of Technology  FAX:   0041-1-632-12-13
> Universitaetstr.16
> CH-8092 Zurich
> "Some ideas are obscure not because they are complex, but because they 
>  are excluded from our circle of comprehension" - Kozma Prutkov
> 
> 

Interesting!! How do you COVALENTLY bind the gel to the glass plate?! I 
would surely appreciate a posting of the protocol.
			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
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