E. coli in microtiter plates
crasmussen at anat.med.ualberta.ca
Wed May 17 18:54:17 EST 1995
rdaines at aol.com (R Daines) wrote:
>Are you shaking them? How many well dishes are these? Try a 20 degree
>slant if volume permits.
We used 96 well plates. What we did was dispense medium containing 20%
glycerol into the plate, innoculated and grew them up. We couldn't
incline them, but this approach solved the poor growth problem, and we
could then send them straight to the -70 freezer without having to add
glycerol to every well. We did about 4000 clones (I think it was that
many) of two different Aspergillus cosmid libraries this way.
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