help for transfection!

Qunfeng Dong qfdong at iastate.edu
Wed May 17 13:06:26 EST 1995


Hi,there:

  I was using the "Modified calcium Phosphate-mediated transfection
procedure" from "Molecular cloning" to transfect chicken muscle cells. After
24 hrs of transfection, most cells look dead, it looks like cell membrane
were just totally disrupted. I don't understand why. Is this because of
Ca2+? The Ca2+ concentration in the media was 6.25 mM/ml.

  Any suggestion?

Qunfeng.



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