help for transfection!

[Qunfeng Dong]

Hi,there:

  I was using the "Modified calcium Phosphate-mediated transfection
procedure" from "Molecular cloning" to transfect chicken muscle cells. After
24 hrs of transfection, most cells look dead, it looks like cell membrane
were just totally disrupted. I don't understand why. Is this because of
Ca2+? The Ca2+ concentration in the media was 6.25 mM/ml.

  Any suggestion?

Qunfeng.