LOW YIELD of mRNA BY THE USE OF MAGNETIC PARTICLES

[Massimo Delledonne]

xz at KEPLER.UNH.EDU (Xin-Hua Zhou) wrote:
>Hi, netter:
>  I have been using the Promega mRNA Islation System. Recently, less than 
>1 ug of mRNA was obtained from about 1000 ug total RNA even though they 
>claim ....... I know my RNA looks good to me, based on the denaturing 
>gel. I am very anxious to know the tricks invloved since everthing such 
>as 32P and materials is ready for cDNA library building. If you have some 
>experience with that, please share yours with me. I really appreciate 
>your suggestions. Thank you!
>
>Zhou

It's sound not so bad ... for what I know, 0.1% of polyA RNA is a normal 
level .....
Anyway that is extactly the efficency I have using that kit (never tried 
something else anyway ..)

Massimo Delledonne
sc2.salk.edu (until december, otherwhise delle at pc.unicatt.it)