RNA Extractions from Liver
Dr Saleem Mohammed
saleem at rfhsm.ac.uk
Wed May 17 21:53:40 EST 1995
Just a quick note, to all you people trying to isolate RNA from Liver. I
have had quite a bit of experience with this and have used all the current
First and foremost the best method I have used which always give's superb
quality RNA and good yields is the CsCl method. Although time consuming, it
is my method of choice for liver.
Methods such as RNAzol and Trizol although OK, do not remove all proteins
resulting in 'dirty RNA' on formaldehyde gels. These reagents are much more
suited to non tissue isolations such as cell culture work.
The use of Cscl involves no use of phenol/chloroform, so resulting in less
sample handling and subsequent loss of RNA.
If anybody wants this 'old method' protocol, please mail me directly. It
usually takes me about 4hrs to isolate such RNA and the quality is great for
Northerns, RNAse protections, reverse transcriptions etc.
Dr Saleem Mohammed,
Dept of Biochemistry & Molecular Biology,
Royal Free Hospital School of Medicine.
Rowland Hill Street,
London. NW2 2PF.
More information about the Methods