barrym at stella.med.utah.edu
Thu May 18 18:05:36 EST 1995
I have been using a Beckman DU-64 spectrophotometer to quantify
concentrations of DNA suspended in H2O or TE. I expect and sometimes
get a 260/280 ratio of 1.8 for pure DNA, and when the ration is
around 2.0 I suspect the presence of RNA. However, I have not been
able to find an explination for the occasional 260/280 readings of
2.5-3.0. What type of contamination or situation would elevate the
260/280 ratio above 2.0?
Thanks for any comments that you might have.
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